ELISA - Enzyme-linked Immunosorbent Assay Solution

Simplify and optimize the ELISA experiment process, providing full process support from sample collection and incubation to washing and result detection. Through precise instruments and equipment, we ensure accurate sample handling and detection, enhance the efficiency of the experiment and the reliability of the results, and make every step of the experiment more convenient and precise.

Background of the Study

Enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical assay that uses antibodies to bind and determine the target molecule in a qualitative or quantitative manner.ELISA methods mainly include direct, indirect, sandwich and competitive methods.Enzyme-linked immunosorbent assay (ELISA) has been used as a diagnostic tool in medicine and plant pathology, as well as the gold standard for quality control checks in various industries, and is widely used in various research fields such as biology and medicine.

Experimental highlights:

  • Well-frozen and active cells
  • Optimize the sample crushing procedure
  • Attention to DNA/RNA purification and concentration
  • Avoiding RNase and DNase contamination
  • Temperature control affects experimental progress

Honghong ELISA experiment full flow program

Sample collection and storage

Samples are obtained and stored at low temperature, and are processed by precise pipetting.

Sample Incubation

The samples were processed according to the experimental requirements and incubated in containers.

purge

Add washing-up liquid and let stand to absorb excess water.

Testing and Analyzing Results

Measurement of OD value using an enzyme marker

Product Details: Sample Collection and Storage

We provide you with high quality instruments for ELISA process. Click “Program Details”You can go to the corresponding product page for detailed information.

DC-CNT-08VT Smart Personalized Centrifuge

DC-CNT-08VT 智能個人離心機
  • Brushless DC motor is ultra-quiet and maintenance-free.
  • Microprocessor controlled for +/- 25 RPM accuracy under variable load conditions.
  • Enhanced airflow design with 8 closed rotors to keep the room temperature within the control range.
  • Minimized footprint and durable construction
  • Suitable for microfiltration, cell separation, centrifuge sedimentation and centrifugation.

DF Series Ultra Low Temperature Refrigerator

DF系列超低溫冰箱
  • Rated power 1600w
  • Separate compartment with insulated door reduces air ingress
  • Powerful and reliable N-Smart™ programmable microprocessor control system with temperature alarm range of ±5°C to ±25°C.
  • Password Protected Control System + High Temperature Failure Alarm + Temperature Sensor Failure Alarm + Door Close Alarm
  • Optional CO2 backup system for extended protection in case of failure

Honghong Bioelectric Pipettes

宏虹生物電動移液器
  • Brushless DC motor is ultra-quiet and maintenance-free.
  • Top-of-the-line stepper motors for unmatched precision and reliability
  • Individual auto-sensing magnetic pistons ensure precise pipetting.
  • 3-point accurate auto-calibration
  • Ultra-efficient battery, up to 5 hours of continuous use after 2 hours of charging
  • Extremely lightweight and painless after a large number of pipetting operations

SampleSafe Series Sample Protectors

SampleSafe 系列樣品保護箱
  • High price/performance ratio: extended sample life at low/high temperatures
  • Effective protection: Dense insulation layer protects samples effectively
  • Safety and Durability: Durable polymer housing, rust and moisture resistant

Product Details: Sample Incubation

We provide you with high quality instruments for ELISA process. Click “Program Details”You can go to the corresponding product page for detailed information.

DC-SK-MP1 Microplate Shaker

  • Built-in counter ensures stable operation and peace of mind.
  • With downward-facing switch plate for easy loading and unloading without leakage
  • Small footprint, can also be used in freezers/incubators (up to 40°C)
  • Two sets of interchangeable fixtures for different microplates
  • Brushless DC motors are quiet, maintenance-free and have a long service life.

PMS-1000i Microplate Shaker

  • Adjustable speed: 150 ~ 1200rpm
  • Temperature of working environment: +4℃ ~ 40℃.
  • Fast and simple screwing of multiwell plates with arbitrary standard depths
  • Gentle oscillation ensures that the sample remains in place in the pore, effectively increasing the specific surface area.
  • Continuous operation, or timed switch-off

PHMP Series Microplate Thermostats

PHMP系列 微孔板恒溫振盪器
  • Oscillation rate: 250 ~ 1200 rpm
  • Temperature setting range: +25 ~ 60/100 ℃; Temperature control range: 5 ~ 60/100 ℃ above room temperature
  • 2 capacity models: 2 microplates / 4 microplates
    Bi-directional heating system (heating plate and cover)
  • Temperature calibration adjusts for temperature differences between microtubes and samples.
  • “Soft-start” and “slow-stop” mechanisms for effective sample protection.

Product Details: Cleaning

We provide you with high quality instruments for ELISA process. Click “Program Details”You can go to the corresponding product page for detailed information.

FTA-2i Waste Trap

  • Built-in miniature vacuum pump, generates -200 ~ -800 adjustable negative pressure, sucks up the liquid left on the wall of the tube.
  • Capable of retaining particles larger than 027um, with an efficiency rate of 99.9%.
  • 2L high-temperature and high-pressure polypropylene collection bottle, suitable for the collection of reaction vessel or microplate waste liquid.
  • Vacuum control knob for smooth adjustment of the optimum suction speed.

C-Series Pipettes

生物C系列移液器
  • Superior performance: Durable tip cones made of PVDF (polyvinylidene fluoride) are highly resistant to physical and chemical corrosion and are suitable for a wide range of nozzles.
  • Easy to operate: can be easily pushed with one hand to avoid contamination; easy to use, can be pipetted for a long time
  • Thoughtful design: two-step plunger operation for forward and reverse pipetting techniques
  • Easy identification: each pipette is labeled with a separate permanent serial number

Honghong will provide you with any support you need!

Our professional Honghong team will be the first to respond and provide you with the best service to solve all your problems.