DNA/RNA Manual Extraction Solutions
Guarantee stable and repeatable standardized experiments
Background of the Study
Commonly used manual DNA/RNA extraction methods include adsorbent extraction, magnetic particle extraction, and column extraction. All extraction methods contain common steps: lysis, binding to a carrier (beads, adsorbents or other carriers), washing, and elution.
Experimental highlights:
- Well-frozen and active cells
- Optimize the sample crushing procedure
- Attention to DNA/RNA purification and concentration
- Avoiding RNase and DNase contamination
- Temperature control affects experimental progress
Honghong DNA/RNA Manual Extraction Solution
Reagent resuspension and centrifugation of disposable tubes
Selection of the appropriate centrifuge for the sample to be operated in order to prepare the sample for the next steps.
wash off
Separate the nucleic acid from the carrier and release the target into the appropriate solution.
Sample Cracking
This step is used to release nucleic acids, lysing the cell and damaging the structure of the cell or tissue.
purge
Removes cellular debris, contaminants, impurities and all irrelevant content by washing.
Product Details: Reagent Resuspension and Disposable Tube Centrifugation
We provide you with high quality instruments for DNA/RNA manual extraction experiments, click “Program Details”You can go to the corresponding product page for detailed information.
Microspin 12 Miniature High-Speed Centrifuge
- Rotation speed 100 ~ 14500rpm, the maximum speed reaches about 15s after startup; centrifugal force: 12400g
- Aluminum fixed angle rotor accommodates 12 x 1.5/2.0ml microtubes, 0.2ml and 0.5ml microtube adapters are standard.
- Constant airflow around the rotor reduces the threat of overheating during sample centrifugation.
- Brushless motors run quietly
- Operating temperature is +4 ~ +25℃, can be used in cold room.
DC-CNT-R44 Miniature High Speed Freezing Centrifuge
- DC-CNT-R44 Miniature High Speed Freezing Centrifuge
- Temperature range: -20°C to 40°C, fast cooling available
- Speed Adjustment Range: 500-15000 RPM (RCF 22388 g)
- RPM / RCF can be switched at the touch of a button.
- Temporary centrifugation function for fast centrifugation and pelletizing.
- Provides electronic imbalance detection and emergency cut-off function to ensure the safety of use.
- Built-in condensate drain for easy removal of water droplets without disassembling the rotor
- Low height for easy loading and unloading of samples
LMC-3000 Table Type Low Speed Centrifuge
- Rotation speed: Microtubes up to 3000rpm, Microplates up to 2000rpm
- Timer function: 1 ~ 90 minutes, auto shut-off function
- The rotor has “soft start” and “slow stop” functions for quick and easy replacement.
- Maximum capacity of 12 tubes or 2 microplates
- Multiple acceleration and deceleration modes
LMC-4200R Table-Type Refrigerated Centrifuge
- Rotation speed: up to 4200rpm for microtubes and 2000rpm for microplates.
- Temperature setting range: -10°C to +25°C
- The rotor can be “soft-started” and “slow-stopped” for quick and easy changeover, and can accommodate up to 12 tubes or 2 microplates.
- High acceleration: 15s acceleration to max speed, 30s deceleration to 0
- Timed and automatic shutdown function, automatic “imbalance” detection
NF3000R Large Capacity Refrigerated Tabletop Centrifuge
- 4x750ml large capacity, suitable for a variety of laboratory tubes
- Intelligent control system for easy operation
- Programmable temperature range: -9°C to 40°C to protect the biological activity of the sample.
- Maintain a stable temperature of 4°C at the highest speed of the sample.
- Supports ON-Mobile™ and NuveControl™ Wireless Remote Services
Product Details: Sample Cracking
We provide you with high quality instruments for DNA/RNA manual extraction experiments, click “Program Details”You can go to the corresponding product page for detailed information.
BTD Fixed Module Dryer
- Temperature range: room temperature +5 ~ 100 ºC, rapid heating
- Stability: ±1 °C; Uniformity: ±0.1 ºC
- Built-in timer, digital temperature control to ensure the best accuracy
- Up to 49 microtubes in 4 models, including 24 x 1.5/2.0 ml, 15 x 0.5 ml, 10 x 0.2 ml.
- Plastic housing is sturdy, durable and easy to clean; compact design with small footprint.
QB Series Dryer
- Accurate and reproducible temperature control for fast and safe sample heating.
- The combination of advanced temperature control technology and high quality precision machining ensures optimal thermal conductivity.
- Various models of replaceable modules are available for selection and come standard with a puller for easy and safe module replacement.
- Over-temperature protection keeps users away from danger; optional safety cover protects samples from contamination.
- Timer device with buzzer function for reaction timing, run timing, and delayed on/off of heater
DC-DRY-1 Dry Bath
- Drying heating block temperature adjustment range: room temperature +5 ℃ to 120 ℃, can be quickly heated, temperature uniformity
- The body is made of composite material with high chemical resistance, and the lid is used for protection and insulation.
- Can be used in water or bead baths
- NTC1000K Temperature Probe, Accurate Temperature Measurement
Wide range of tube capacity, easy to pick up and put down, easy to clean and sterilize.
PHMT Series Constant Temperature Oscillator
- Oscillation rate: 250 ~ 1400 rpm
- Temperature setting range: +25 ~ 100 ℃; Temperature control range: 5 ~ 100 ℃ on room temperature
- Holds up to 24 or 32 microtubes or 1 96-well microplate
- Temperature control and mixing functions can be combined or used individually, combining mixing and incubation phases to reduce reaction time; five interchangeable and adaptable heating blocks are available
- Temperature calibration function to adjust the temperature difference between different microtubes and samples.
- “Soft start” function for precision samples
PCMT Coolable Constant Temperature Oscillator
- Oscillation rate: 250 ~ 1400 rpm
- Temperature setting range: +4 ~ 100 °C
- Temperature control range: 15 ~ 100 ℃ at room temperature; Temperature stability: ±1 ℃.
- Combined or individual use of temperature control (heating/cooling) and mixing functions
- Temperature calibration function to adjust the temperature difference between different microtubes and samples.
- Choice of five interchangeable and adaptable heating blocks
Product Details: Cleaning
We provide you with high quality instruments for DNA/RNA manual extraction experiments, click “Program Details”You can go to the corresponding product page for detailed information.
FTA-2i Waste Trap
- Built-in miniature vacuum pump, generates -200 ~ -800 adjustable negative pressure, sucks up the liquid left on the wall of the tube.
- Capable of retaining particles larger than 027um, with an efficiency rate of 99.9%.
- 2L high-temperature and high-pressure polypropylene collection bottle, suitable for the collection of reaction vessel or microplate waste liquid.
- Vacuum control knob for smooth adjustment of the optimum suction speed.
J-Series Pipettes
- 130mm, compact size, no fatigue operation
- Two-step plunger operation for “reverse pipetting technique”.”
- Fully autoclavable body for easy sterilization
- Tip cone designed for -10µI and 200µI tips for ease of use
- Stainless steel piston for reliable performance and durability
- Customizable in any capacity and color combination between 2µI and 100µI
C-Series Pipettes
- Superior performance: Durable tip cones made of PVDF (polyvinylidene fluoride) are highly resistant to physical and chemical corrosion and are suitable for a wide range of nozzles.
- Easy to operate: can be easily pushed with one hand to avoid contamination; easy to use, can be pipetted for a long time
- Thoughtful design: two-step plunger operation for forward and reverse pipetting techniques
- Easy identification: each pipette is labeled with a separate permanent serial number
Product Details: Wash off
We provide you with high quality instruments for DNA/RNA manual extraction experiments, click “Program Details”You can go to the corresponding product page for detailed information.
DC-CNT-M12 High Speed Microcentrifuge
- Maximum rotor capacity 12 x 1.5/2.0 ml
- Small footprint saves valuable bench space.
- Intelligent design for smooth and quiet operation even at maximum speeds
- Dual shell design and tried and tested explosion-proof structural design
- Safe Low Voltage Device (24V) with Universal Power Supply
PCH Series Heating/Cooling Dryers
- Heating/cooling temperature range: -10 ~ 100 ℃, stability: ±1 ℃.
- Unique component construction combined with Peltier coolers for rapid heating and cooling
- Rapidly achieves DNA denaturation temperature: 95℃.
- PCH-1 can accommodate 32 microtubes in 2 sizes; PCH-2 can accommodate 20 microtubes in 1 size; PCH-3 can accommodate 20 microtubes in 1 size.
PHMP Series Microplate Thermostats
- Oscillation rate: 250 ~ 1200 rpm
- Temperature setting range: +25 ~ 60/100 ℃; Temperature control range: 5 ~ 60/100 ℃ above room temperature
- 2 capacity models: 2 microplates / 4 microplates
Bi-directional heating system (heating plate and cover) - Temperature calibration adjusts for temperature differences between microtubes and samples.
- “Soft-start” and “slow-stop” mechanisms for effective sample protection.
PCMT PLUS Coolable Constant Temperature Oscillator
- Powerful mixing and temperature control of samples in microtubes or PCR plates
- Equipped with cooling function, 7 devices can be controlled from PC via Bluetooth connection.
- Powerful software features to meet experimental needs
- Heating and mixing functions can run simultaneously or independently.
- There are five heating and cooling blocks available.
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