{"id":31030,"date":"2023-05-03T10:16:40","date_gmt":"2023-05-03T02:16:40","guid":{"rendered":"https:\/\/hongtronics.com\/?p=31030"},"modified":"2026-09-15T14:05:51","modified_gmt":"2026-09-15T06:05:51","slug":"article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation","status":"publish","type":"post","link":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/","title":{"rendered":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"31030\" class=\"elementor elementor-31030\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e110d35 e-flex e-con-boxed e-con e-parent\" data-id=\"e110d35\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-114ad44 elementor-widget__width-initial elementor-widget elementor-widget-html\" data-id=\"114ad44\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<article class=\"hh-seo-article\">\r\n\r\n  <header class=\"hh-seo-article__intro\">\r\n    <div class=\"hh-seo-article__eyebrow\">\r\n      Cell Culture Workflow Guide\r\n    <\/div>\r\n\r\n    <h2 class=\"hh-seo-article__intro-title\">\r\n      \u5f9e\u7d30\u80de\u5fa9\u7526\u5230\u51b7\u51cd\u4fdd\u5b58\uff1a\u638c\u63e1\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6838\u5fc3\u6b65\u9a5f\r\n    <\/h2>\r\n\r\n    <p class=\"hh-seo-article__intro-lead\">\r\n      \u7d30\u80de\u57f9\u990a\u53ef\u6982\u62ec\u70ba\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u4e09\u500b\u6838\u5fc3\u968e\u6bb5\uff0c\u5206\u5225\u8ca0\u8cac\u6062\u5fa9\u51b7\u51cd\u7d30\u80de\u3001\u7dad\u6301\u7d30\u80de\u7a69\u5b9a\u589e\u6b96\uff0c\u4ee5\u53ca\u5efa\u7acb\u53ef\u9577\u671f\u4f7f\u7528\u7684\u7d30\u80de\u5eab\u3002\r\n    <\/p>\r\n\r\n    <p class=\"hh-seo-article__intro-text\">\r\n      \u6bcf\u7a2e\u7d30\u80de\u682a\u5c0d\u57f9\u990a\u57fa\u3001\u89e3\u96e2\u65b9\u5f0f\u3001\u63a5\u7a2e\u5bc6\u5ea6\u3001\u878d\u5408\u5ea6\u8207\u964d\u6eab\u689d\u4ef6\u7684\u9700\u6c42\u90fd\u53ef\u80fd\u4e0d\u540c\u3002\u672c\u6587\u63d0\u4f9b\u7684\u662f\u901a\u7528\u539f\u5247\u8207\u6d41\u7a0b\u67b6\u69cb\uff0c\u5be6\u969b\u64cd\u4f5c\u4ecd\u61c9\u512a\u5148\u4f9d\u5faa\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u3001\u7d30\u80de\u5eab\u6307\u5f15\u53ca\u5be6\u9a57\u5ba4 SOP\u3002\r\n    <\/p>\r\n\r\n    <ol class=\"hh-seo-article__summary\" aria-label=\"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\">\r\n      <li>\r\n        <strong>01\uff5c\u7d30\u80de\u5fa9\u7526<\/strong>\r\n        <span>\u5feb\u901f\u4e14\u5b89\u5168\u5730\u89e3\u51cd\u51b7\u51cd\u7d30\u80de<\/span>\r\n      <\/li>\r\n      <li>\r\n        <strong>02\uff5c\u7e7c\u4ee3\u57f9\u990a<\/strong>\r\n        <span>\u7dad\u6301\u9069\u7576\u5bc6\u5ea6\u8207\u5065\u5eb7\u751f\u9577\u72c0\u614b<\/span>\r\n      <\/li>\r\n      <li>\r\n        <strong>03\uff5c\u51b7\u51cd\u4fdd\u5b58<\/strong>\r\n        <span>\u5efa\u7acb\u7a69\u5b9a\u4e14\u53ef\u8ffd\u6eaf\u7684\u7d30\u80de\u5eab<\/span>\r\n      <\/li>\r\n    <\/ol>\r\n  <\/header>\r\n\r\n  <figure class=\"hh-seo-article__figure hh-seo-article__figure--hero\">\r\n    <img fetchpriority=\"high\"\r\n      src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12.png\"\r\n      alt=\"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u8207 Grant \u7a0b\u5e8f\u964d\u6eab\u5100\"\r\n      width=\"700\"\r\n      height=\"700\"\r\n      decoding=\"async\">\r\n    <figcaption>\r\n      \u7d30\u80de\u57f9\u990a\u7684\u6838\u5fc3\u6d41\u7a0b\u6db5\u84cb\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u3002\r\n    <\/figcaption>\r\n  <\/figure>\r\n\r\n  <nav class=\"hh-seo-article__toc\" aria-labelledby=\"hh-cell-toc-title\">\r\n    <div class=\"hh-seo-article__toc-label\" id=\"hh-cell-toc-title\">\r\n      \u6587\u7ae0\u5c0e\u89bd\r\n    <\/div>\r\n\r\n    <ol>\r\n      <li><a href=\"#cell-culture-overview\">\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f<\/a><\/li>\r\n      <li><a href=\"#preparation\">\u57f9\u990a\u524d\u6e96\u5099\u689d\u4ef6<\/a><\/li>\r\n      <li><a href=\"#cell-thawing\">\u7b2c\u4e00\u6b65\uff1a\u7d30\u80de\u5fa9\u7526<\/a><\/li>\r\n      <li><a href=\"#cell-passaging\">\u7b2c\u4e8c\u6b65\uff1a\u7e7c\u4ee3\u57f9\u990a<\/a><\/li>\r\n      <li><a href=\"#cell-cryopreservation\">\u7b2c\u4e09\u6b65\uff1a\u51b7\u51cd\u4fdd\u5b58<\/a><\/li>\r\n      <li><a href=\"#post-thaw-quality\">\u89e3\u51cd\u5f8c\u54c1\u8cea\u6aa2\u67e5<\/a><\/li>\r\n      <li><a href=\"#equipment-selection\">\u8a2d\u5099\u9078\u64c7\u91cd\u9ede<\/a><\/li>\r\n      <li><a href=\"#microfluidics\">\u5fae\u6d41\u63a7\u7814\u7a76\u5ef6\u4f38<\/a><\/li>\r\n      <li><a href=\"#frequently-asked-questions\">Frequently Asked Questions<\/a><\/li>\r\n    <\/ol>\r\n  <\/nav>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"cell-culture-overview\">\r\n    <div class=\"hh-seo-article__section-label\">Workflow Overview<\/div>\r\n\r\n    <h2>\u7d30\u80de\u57f9\u990a\u5305\u542b\u54ea\u4e09\u500b\u6838\u5fc3\u6b65\u9a5f\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u7d30\u80de\u57f9\u990a\u7684\u4e09\u500b\u6838\u5fc3\u6b65\u9a5f\u662f\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u3002\u4e09\u8005\u5f62\u6210\u4e00\u500b\u5faa\u74b0\uff1a\u5f9e\u7d30\u80de\u5eab\u53d6\u51fa\u4e26\u5fa9\u7526\u7d30\u80de\uff0c\u7d93\u57f9\u990a\u8207\u7e7c\u4ee3\u64f4\u589e\u5f8c\uff0c\u518d\u5c07\u72c0\u614b\u5408\u9069\u7684\u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u3002\r\n    <\/p>\r\n\r\n    <p>\r\n      \u7d30\u80de\u57f9\u990a\u662f\u6307\u5728\u9ad4\u5916\u63d0\u4f9b\u9069\u7576\u7684\u57f9\u990a\u57fa\u3001\u6eab\u5ea6\u3001\u6c23\u9ad4\u3001\u9178\u9e7c\u503c\u8207\u7121\u83cc\u74b0\u5883\uff0c\u4f7f\u7d30\u80de\u7dad\u6301\u5b58\u6d3b\u3001\u751f\u9577\u6216\u7279\u5b9a\u529f\u80fd\u3002\u7814\u7a76\u4eba\u54e1\u53ef\u85c9\u6b64\u89c0\u5bdf\u7d30\u80de\u578b\u614b\u3001\u589e\u6b96\u3001\u4ee3\u8b1d\u3001\u8a0a\u865f\u50b3\u905e\u53ca\u85e5\u7269\u53cd\u61c9\u3002\r\n    <\/p>\r\n\r\n    <div\r\n      class=\"hh-seo-article__table-wrap\"\r\n      tabindex=\"0\"\r\n      aria-label=\"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u6bd4\u8f03\u8868\uff0c\u53ef\u6c34\u5e73\u6372\u52d5\">\r\n\r\n      <table>\r\n        <caption>\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u6bd4\u8f03<\/caption>\r\n\r\n        <thead>\r\n          <tr>\r\n            <th scope=\"col\">\u968e\u6bb5<\/th>\r\n            <th scope=\"col\">\u4e3b\u8981\u76ee\u7684<\/th>\r\n            <th scope=\"col\">\u6838\u5fc3\u539f\u5247<\/th>\r\n            <th scope=\"col\">\u89c0\u5bdf\u91cd\u9ede<\/th>\r\n          <\/tr>\r\n        <\/thead>\r\n\r\n        <tbody>\r\n          <tr>\r\n            <th scope=\"row\">\u7d30\u80de\u5fa9\u7526<\/th>\r\n            <td>\u8b93\u51b7\u51cd\u7d30\u80de\u6062\u5fa9\u57f9\u990a<\/td>\r\n            <td>\u4f9d\u6307\u5f15\u5feb\u901f\u89e3\u51cd\u4e26\u964d\u4f4e\u51b7\u51cd\u4fdd\u5b58\u6db2\u6301\u7e8c\u66b4\u9732<\/td>\r\n            <td>\u5b58\u6d3b\u7387\u3001\u8cbc\u9644\u3001\u578b\u614b\u8207\u521d\u671f\u6062\u5fa9<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u7e7c\u4ee3\u57f9\u990a<\/th>\r\n            <td>\u7dad\u6301\u7d30\u80de\u751f\u9577\u4e26\u64f4\u589e\u6578\u91cf<\/td>\r\n            <td>\u5728\u9069\u7576\u6642\u6a5f\u89e3\u96e2\u3001\u8a08\u6578\u53ca\u91cd\u65b0\u63a5\u7a2e<\/td>\r\n            <td>\u878d\u5408\u5ea6\u3001\u7d30\u80de\u578b\u614b\u3001\u751f\u9577\u901f\u5ea6\u8207\u6c59\u67d3<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u51b7\u51cd\u4fdd\u5b58<\/th>\r\n            <td>\u5efa\u7acb\u7a2e\u6e90\u7d30\u80de\u5eab\u6216\u5de5\u4f5c\u7d30\u80de\u5eab<\/td>\r\n            <td>\u4f7f\u7528\u5408\u9069\u51b7\u51cd\u4fdd\u5b58\u6db2\u4e26\u63a7\u5236\u964d\u6eab\u904e\u7a0b<\/td>\r\n            <td>\u7d30\u80de\u72c0\u614b\u3001\u6fc3\u5ea6\u3001\u964d\u6eab\u7d00\u9304\u8207\u5132\u5b58\u4f4d\u7f6e<\/td>\r\n          <\/tr>\r\n        <\/tbody>\r\n      <\/table>\r\n    <\/div>\r\n\r\n    <aside class=\"hh-seo-article__note\">\r\n      <strong>\u672c\u6587\u4e0d\u662f\u6240\u6709\u7d30\u80de\u682a\u7684\u56fa\u5b9a SOP<\/strong>\r\n      <p>\r\n        \u539f\u4ee3\u7d30\u80de\u3001\u816b\u7624\u7d30\u80de\u682a\u3001\u5e79\u7d30\u80de\u3001\u8cbc\u9644\u7d30\u80de\u8207\u61f8\u6d6e\u7d30\u80de\u7684\u64cd\u4f5c\u689d\u4ef6\u53ef\u80fd\u4e0d\u540c\u3002\u9032\u884c\u5be6\u9a57\u524d\uff0c\u61c9\u5148\u78ba\u8a8d\u7d30\u80de\u4f86\u6e90\u63d0\u4f9b\u7684\u5c08\u5c6c\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u8aaa\u660e\u3002\r\n      <\/p>\r\n    <\/aside>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"preparation\">\r\n    <div class=\"hh-seo-article__section-label\">Before You Start<\/div>\r\n\r\n    <h2>\u958b\u59cb\u7d30\u80de\u57f9\u990a\u524d\uff0c\u9700\u8981\u5148\u78ba\u8a8d\u54ea\u4e9b\u689d\u4ef6\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u958b\u59cb\u7d30\u80de\u57f9\u990a\u524d\uff0c\u61c9\u5148\u78ba\u8a8d\u7d30\u80de\u682a\u8eab\u5206\u3001\u57f9\u990a\u689d\u4ef6\u3001\u57f9\u990a\u57fa\u3001\u7121\u83cc\u8a2d\u5099\u8207\u5b89\u5168\u898f\u7bc4\u3002\u82e5\u524d\u7f6e\u8cc7\u8a0a\u4e0d\u5b8c\u6574\uff0c\u5373\u4f7f\u64cd\u4f5c\u6b65\u9a5f\u6b63\u78ba\uff0c\u4e5f\u53ef\u80fd\u9020\u6210\u7d30\u80de\u6062\u5fa9\u4e0d\u826f\u3001\u578b\u614b\u6539\u8b8a\u6216\u5be6\u9a57\u7d50\u679c\u7121\u6cd5\u91cd\u73fe\u3002\r\n    <\/p>\r\n\r\n    <div class=\"hh-seo-article__check-grid\">\r\n      <section>\r\n        <span>01<\/span>\r\n        <h3>\u7d30\u80de\u682a\u8aaa\u660e<\/h3>\r\n        <p>\u78ba\u8a8d\u7d30\u80de\u4f86\u6e90\u3001\u7269\u7a2e\u3001\u7d44\u7e54\u3001\u57f9\u990a\u578b\u614b\u3001\u5efa\u8b70\u57f9\u990a\u57fa\u8207\u7e7c\u4ee3\u65b9\u5f0f\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>02<\/span>\r\n        <h3>\u57f9\u990a\u74b0\u5883<\/h3>\r\n        <p>\u78ba\u8a8d\u57f9\u990a\u6eab\u5ea6\u3001\u6c23\u9ad4\u689d\u4ef6\u3001\u6fd5\u5ea6\u8207\u57f9\u990a\u5bb9\u5668\u662f\u5426\u7b26\u5408\u9700\u6c42\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>03<\/span>\r\n        <h3>\u57f9\u990a\u57fa\u8207\u8a66\u5291<\/h3>\r\n        <p>\u6e96\u5099\u5b8c\u6574\u57f9\u990a\u57fa\u3001\u6dfb\u52a0\u7269\u3001\u7de9\u885d\u6db2\u53ca\u9069\u5408\u7684\u7d30\u80de\u89e3\u96e2\u8a66\u5291\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>04<\/span>\r\n        <h3>\u7121\u83cc\u64cd\u4f5c<\/h3>\r\n        <p>\u78ba\u8a8d\u751f\u7269\u5b89\u5168\u64cd\u4f5c\u6ac3\u3001\u5668\u6750\u3001\u8017\u6750\u8207\u5de5\u4f5c\u5340\u57df\u5df2\u5b8c\u6210\u6e05\u6f54\u8207\u6e96\u5099\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>05<\/span>\r\n        <h3>\u54c1\u8cea\u76e3\u6e2c<\/h3>\r\n        <p>\u5efa\u7acb\u7d30\u80de\u578b\u614b\u3001\u5b58\u6d3b\u7387\u3001\u6c59\u67d3\u3001\u9ef4\u6f3f\u83cc\u8207\u7e7c\u4ee3\u6b21\u6578\u7684\u6aa2\u67e5\u7d00\u9304\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>06<\/span>\r\n        <h3>\u4eba\u54e1\u5b89\u5168<\/h3>\r\n        <p>\u4f9d\u751f\u7269\u5b89\u5168\u7b49\u7d1a\u53ca\u6db2\u614b\u6c2e\u4f7f\u7528\u898f\u7bc4\uff0c\u6e96\u5099\u5fc5\u8981\u7684\u500b\u4eba\u9632\u8b77\u88dd\u5099\u3002<\/p>\r\n      <\/section>\r\n    <\/div>\r\n\r\n    <p>\r\n      \u82e5\u9700\u8981\u6574\u5408\u57f9\u990a\u3001\u96e2\u5fc3\u3001\u6df7\u5408\u3001\u6eab\u63a7\u8207\u51b7\u51cd\u4fdd\u5b58\u8a2d\u5099\uff0c\u53ef\u9032\u4e00\u6b65\u53c3\u8003\u5b8f\u8679\u7684\r\n      <a href=\"https:\/\/hongtronics.com\/en\/cell-lab-solutions-com\/\">\r\n        Integrated Cell Laboratory Systems\r\n      <\/a>\u3002\r\n    <\/p>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"cell-thawing\">\r\n    <div class=\"hh-seo-article__section-label\">Step 01<\/div>\r\n\r\n    <h2>\u7b2c\u4e00\u6b65\uff1a\u7d30\u80de\u5fa9\u7526\u70ba\u4ec0\u9ebc\u9700\u8981\u5feb\u901f\u89e3\u51cd\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u7d30\u80de\u5fa9\u7526\u901a\u5e38\u63a1\u5feb\u901f\u89e3\u51cd\uff0c\u76ee\u7684\u662f\u7e2e\u77ed\u7d30\u80de\u8655\u65bc\u4e0d\u5229\u6eab\u5ea6\u5340\u9593\u53ca\u542b\u51b7\u51cd\u4fdd\u8b77\u5291\u74b0\u5883\u4e2d\u7684\u6642\u9593\u3002\u5e38\u898b\u901a\u7528\u65b9\u6cd5\u662f\u5c07\u51b7\u51cd\u7ba1\u7f6e\u65bc\u7d04 37\u00b0C \u6c34\u6d74\u4e2d\u8fc5\u901f\u89e3\u51cd\uff0c\u4f46\u4ecd\u9808\u4f9d\u7d30\u80de\u682a\u5c08\u5c6c\u6307\u5f15\u8abf\u6574\u3002\r\n    <\/p>\r\n\r\n    <p>\r\n      \u89e3\u51cd\u4e26\u4e0d\u662f\u55ae\u7d14\u5c07\u51b7\u51cd\u7ba1\u5347\u6eab\u3002\u7814\u7a76\u4eba\u54e1\u9084\u9700\u63a7\u5236\u64cd\u4f5c\u6642\u9593\u3001\u51b7\u51cd\u4fdd\u5b58\u6db2\u7684\u7a00\u91cb\u6216\u79fb\u9664\u65b9\u5f0f\u3001\u57f9\u990a\u57fa\u72c0\u614b\uff0c\u4ee5\u53ca\u63a5\u7a2e\u5f8c\u7684\u6062\u5fa9\u689d\u4ef6\u3002\r\n    <\/p>\r\n\r\n    <h3>\u7d30\u80de\u5fa9\u7526\u7684\u901a\u7528\u6d41\u7a0b<\/h3>\r\n\r\n    <ol class=\"hh-seo-article__steps\">\r\n      <li>\r\n        <div>\r\n          <strong>\u78ba\u8a8d\u7d30\u80de\u682a\u8207\u57f9\u990a\u689d\u4ef6<\/strong>\r\n          <p>\u6838\u5c0d\u51b7\u51cd\u7ba1\u6a19\u793a\u3001\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u3001\u57f9\u990a\u57fa\u53ca\u9810\u5b9a\u63a5\u7a2e\u5bb9\u5668\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u6e96\u5099\u57f9\u990a\u57fa\u8207\u64cd\u4f5c\u74b0\u5883<\/strong>\r\n          <p>\u4f9d\u7d30\u80de\u682a\u6307\u5f15\u6e96\u5099\u57f9\u990a\u57fa\uff0c\u4e26\u5b8c\u6210\u64cd\u4f5c\u6ac3\u3001\u5668\u6750\u8207\u8017\u6750\u914d\u7f6e\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u8fc5\u901f\u89e3\u51cd\u51b7\u51cd\u7d30\u80de<\/strong>\r\n          <p>\u5c07\u51b7\u51cd\u7ba1\u7f6e\u65bc\u5efa\u8b70\u6eab\u5ea6\u7684\u6c34\u6d74\u4e2d\u8f15\u67d4\u6643\u52d5\uff0c\u907f\u514d\u6574\u500b\u7ba1\u84cb\u6d78\u5165\u6c34\u4e2d\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u6e05\u6f54\u51b7\u51cd\u7ba1\u5916\u90e8<\/strong>\r\n          <p>\u79fb\u5165\u7121\u83cc\u64cd\u4f5c\u5340\u524d\uff0c\u4f9d\u5be6\u9a57\u5ba4\u898f\u7bc4\u6e05\u6f54\u51b7\u51cd\u7ba1\u5916\u90e8\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u8655\u7406\u51b7\u51cd\u4fdd\u5b58\u6db2<\/strong>\r\n          <p>\u4f9d\u7d30\u80de\u682a\u6307\u5f15\u7a00\u91cb\u6216\u79fb\u9664\u51b7\u51cd\u4fdd\u5b58\u6db2\uff0c\u907f\u514d\u4e0d\u5fc5\u8981\u7684\u9577\u6642\u9593\u66b4\u9732\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u63a5\u7a2e\u4e26\u958b\u59cb\u6062\u5fa9\u57f9\u990a<\/strong>\r\n          <p>\u5c07\u7d30\u80de\u8f49\u79fb\u81f3\u5408\u9069\u57f9\u990a\u5bb9\u5668\uff0c\u7f6e\u65bc\u6307\u5b9a\u6eab\u5ea6\u8207\u6c23\u9ad4\u689d\u4ef6\u4e0b\u57f9\u990a\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u89c0\u5bdf\u89e3\u51cd\u5f8c\u72c0\u614b<\/strong>\r\n          <p>\u4f9d\u5efa\u8b70\u6642\u9593\u6aa2\u67e5\u7d30\u80de\u5b58\u6d3b\u3001\u8cbc\u9644\u3001\u578b\u614b\u3001\u6c59\u67d3\u8207\u57f9\u990a\u57fa\u72c0\u614b\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n    <\/ol>\r\n\r\n    <h3>\u7d30\u80de\u5fa9\u7526\u5e38\u898b\u554f\u984c<\/h3>\r\n\r\n    <ul class=\"hh-seo-article__feature-list\">\r\n      <li>\r\n        <strong>\u89e3\u51cd\u901f\u5ea6\u904e\u6162<\/strong>\r\n        <span>\u53ef\u80fd\u5ef6\u9577\u7d30\u80de\u8655\u65bc\u4e0d\u5229\u6eab\u5ea6\u5340\u9593\u7684\u6642\u9593\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u64cd\u4f5c\u904e\u65bc\u5287\u70c8<\/strong>\r\n        <span>\u53cd\u8986\u5f37\u529b\u5439\u6253\u6216\u9ad8\u901f\u8655\u7406\u53ef\u80fd\u589e\u52a0\u7d30\u80de\u53d7\u5230\u7684\u6a5f\u68b0\u6027\u58d3\u529b\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u4fdd\u5b58\u6db2\u8655\u7406\u4e0d\u7576<\/strong>\r\n        <span>\u51b7\u51cd\u4fdd\u8b77\u5291\u7684\u79fb\u9664\u6216\u7a00\u91cb\u65b9\u5f0f\u61c9\u4f9d\u7d30\u80de\u682a\u6307\u5f15\u57f7\u884c\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u63a5\u7a2e\u689d\u4ef6\u4e0d\u5408\u9069<\/strong>\r\n        <span>\u63a5\u7a2e\u5bc6\u5ea6\u3001\u57f9\u990a\u57fa\u3001\u57fa\u8cea\u6216\u5bb9\u5668\u4e0d\u7b26\u9700\u6c42\uff0c\u53ef\u80fd\u5f71\u97ff\u6062\u5fa9\u3002<\/span>\r\n      <\/li>\r\n    <\/ul>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"cell-passaging\">\r\n    <div class=\"hh-seo-article__section-label\">Step 02<\/div>\r\n\r\n    <h2>\u7b2c\u4e8c\u6b65\uff1a\u7d30\u80de\u4f55\u6642\u9700\u8981\u7e7c\u4ee3\u57f9\u990a\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u7d30\u80de\u662f\u5426\u9700\u8981\u7e7c\u4ee3\u57f9\u990a\uff0c\u4e0d\u80fd\u53ea\u4f9d\u56fa\u5b9a\u878d\u5408\u5ea6\u5224\u65b7\u3002\u7814\u7a76\u4eba\u54e1\u61c9\u540c\u6642\u53c3\u8003\u7d30\u80de\u682a\u5efa\u8b70\u3001\u7d30\u80de\u578b\u614b\u3001\u751f\u9577\u901f\u5ea6\u3001\u57f9\u990a\u57fa\u72c0\u614b\u3001\u57f9\u990a\u5929\u6578\u53ca\u5f8c\u7e8c\u5be6\u9a57\u7528\u9014\u3002\r\n    <\/p>\r\n\r\n    <p>\r\n      \u7e7c\u4ee3\u57f9\u990a\u4ea6\u5e38\u88ab\u7a31\u70ba\u7d30\u80de\u50b3\u4ee3\uff0c\u76ee\u7684\u662f\u5c07\u7d30\u80de\u5f9e\u65e2\u6709\u57f9\u990a\u5bb9\u5668\u8f49\u79fb\u81f3\u65b0\u7684\u57f9\u990a\u74b0\u5883\uff0c\u4f7f\u7d30\u80de\u7dad\u6301\u5408\u9069\u5bc6\u5ea6\u4e26\u7e7c\u7e8c\u751f\u9577\u3002\r\n    <\/p>\r\n\r\n    <h3>\u8cbc\u9644\u7d30\u80de\u7684\u901a\u7528\u7e7c\u4ee3\u57f9\u990a\u6d41\u7a0b<\/h3>\r\n\r\n    <ol class=\"hh-seo-article__steps\">\r\n      <li>\r\n        <div>\r\n          <strong>\u89c0\u5bdf\u7d30\u80de\u72c0\u614b<\/strong>\r\n          <p>\u4f7f\u7528\u986f\u5fae\u93e1\u78ba\u8a8d\u878d\u5408\u5ea6\u3001\u7d30\u80de\u578b\u614b\u3001\u57f9\u990a\u57fa\u8207\u53ef\u80fd\u7684\u6c59\u67d3\u60c5\u5f62\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u79fb\u9664\u820a\u57f9\u990a\u57fa<\/strong>\r\n          <p>\u4f9d\u7d30\u80de\u682a\u6d41\u7a0b\u79fb\u9664\u57f9\u990a\u57fa\uff0c\u5fc5\u8981\u6642\u4f7f\u7528\u9069\u5408\u7684\u7de9\u885d\u6db2\u6e05\u6d17\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u9032\u884c\u7d30\u80de\u89e3\u96e2<\/strong>\r\n          <p>\u9078\u7528\u9069\u5408\u7684\u9175\u7d20\u3001\u975e\u9175\u7d20\u8a66\u5291\u6216\u6a5f\u68b0\u65b9\u5f0f\u4f7f\u8cbc\u9644\u7d30\u80de\u5206\u96e2\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u7d42\u6b62\u6216\u7a00\u91cb\u89e3\u96e2\u53cd\u61c9<\/strong>\r\n          <p>\u4f9d\u89e3\u96e2\u8a66\u5291\u53ca\u7d30\u80de\u682a\u8981\u6c42\u52a0\u5165\u57f9\u990a\u57fa\u6216\u6307\u5b9a\u6eb6\u6db2\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u6536\u96c6\u4e26\u8a08\u7b97\u7d30\u80de<\/strong>\r\n          <p>\u4f9d\u5be6\u9a57\u9700\u6c42\u9032\u884c\u96e2\u5fc3\u3001\u91cd\u61f8\u3001\u7d30\u80de\u8a08\u6578\u8207\u5b58\u6d3b\u7387\u8a55\u4f30\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u4f9d\u5efa\u8b70\u5bc6\u5ea6\u91cd\u65b0\u63a5\u7a2e<\/strong>\r\n          <p>\u5c07\u9069\u91cf\u7d30\u80de\u63a5\u7a2e\u81f3\u65b0\u7684\u57f9\u990a\u5bb9\u5668\uff0c\u4e26\u5b8c\u6574\u8a18\u9304\u7e7c\u4ee3\u8cc7\u8a0a\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n    <\/ol>\r\n\r\n    <div class=\"hh-seo-article__compare-grid\">\r\n      <section>\r\n        <span>\u5bc6\u5ea6\u904e\u9ad8<\/span>\r\n        <h3>\u53ef\u80fd\u51fa\u73fe\u7684\u60c5\u5f62<\/h3>\r\n        <ul>\r\n          <li>\u990a\u5206\u8f03\u5feb\u8017\u76e1\u3002<\/li>\r\n          <li>\u4ee3\u8b1d\u5ee2\u7269\u9010\u6f38\u7d2f\u7a4d\u3002<\/li>\r\n          <li>\u7d30\u80de\u578b\u614b\u6216\u751f\u9577\u72c0\u614b\u6539\u8b8a\u3002<\/li>\r\n          <li>\u5f8c\u7e8c\u89e3\u96e2\u8207\u5747\u52fb\u91cd\u61f8\u66f4\u52a0\u56f0\u96e3\u3002<\/li>\r\n          <li>\u5be6\u9a57\u7d50\u679c\u7684\u6279\u6b21\u5dee\u7570\u589e\u52a0\u3002<\/li>\r\n        <\/ul>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>\u5bc6\u5ea6\u904e\u4f4e<\/span>\r\n        <h3>\u53ef\u80fd\u51fa\u73fe\u7684\u60c5\u5f62<\/h3>\r\n        <ul>\r\n          <li>\u7d30\u80de\u8cbc\u9644\u8207\u6062\u5fa9\u901f\u5ea6\u4e0b\u964d\u3002<\/li>\r\n          <li>\u7d30\u80de\u9593\u8a0a\u606f\u8207\u751f\u9577\u74b0\u5883\u4e0d\u8db3\u3002<\/li>\r\n          <li>\u589e\u6b96\u901f\u5ea6\u8b8a\u6162\u3002<\/li>\r\n          <li>\u90e8\u5206\u7d30\u80de\u682a\u7684\u5b58\u6d3b\u7387\u4e0b\u964d\u3002<\/li>\r\n          <li>\u9054\u5230\u5be6\u9a57\u6240\u9700\u7d30\u80de\u91cf\u7684\u6642\u9593\u5ef6\u9577\u3002<\/li>\r\n        <\/ul>\r\n      <\/section>\r\n    <\/div>\r\n\r\n    <aside class=\"hh-seo-article__note\">\r\n      <strong>80% \u878d\u5408\u5ea6\u4e0d\u662f\u6240\u6709\u7d30\u80de\u682a\u7684\u56fa\u5b9a\u7b54\u6848<\/strong>\r\n      <p>\r\n        \u90e8\u5206\u8cbc\u9644\u578b\u7d30\u80de\u53ef\u80fd\u5728\u7d04 70%\uff5e90% \u878d\u5408\u5ea6\u9593\u9032\u884c\u7e7c\u4ee3\uff0c\u4f46\u5e79\u7d30\u80de\u3001\u539f\u4ee3\u7d30\u80de\u53ca\u4e0d\u540c\u816b\u7624\u7d30\u80de\u682a\u53ef\u80fd\u6709\u4e0d\u540c\u6a19\u6e96\u3002\u61c9\u512a\u5148\u63a1\u7528\u7d30\u80de\u4f86\u6e90\u63d0\u4f9b\u7684\u5efa\u8b70\u3002\r\n      <\/p>\r\n    <\/aside>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"cell-cryopreservation\">\r\n    <div class=\"hh-seo-article__section-label\">Step 03<\/div>\r\n\r\n    <h2>\u7b2c\u4e09\u6b65\uff1a\u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u70ba\u4ec0\u9ebc\u9700\u8981\u63a7\u5236\u964d\u6eab\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u9700\u8981\u642d\u914d\u9069\u7576\u7684\u51b7\u51cd\u4fdd\u5b58\u6db2\u8207\u53d7\u63a7\u964d\u6eab\u6d41\u7a0b\uff0c\u4ee5\u964d\u4f4e\u51b7\u51cd\u904e\u7a0b\u4e2d\u6ef2\u900f\u58d3\u8b8a\u5316\u53ca\u51b0\u6676\u5f62\u6210\u5c0d\u7d30\u80de\u9020\u6210\u7684\u640d\u50b7\u3002\u6700\u4f73\u964d\u6eab\u689d\u4ef6\u9808\u4f9d\u7d30\u80de\u7a2e\u985e\u8207\u9a57\u8b49\u7d50\u679c\u8a2d\u5b9a\u3002\r\n    <\/p>\r\n\r\n    <p>\r\n      \u6bcf\u5206\u9418\u7d04 1\u00b0C \u662f\u8a31\u591a\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u5e38\u898b\u7684\u8d77\u59cb\u964d\u6eab\u689d\u4ef6\uff0c\u4f46\u4e0d\u662f\u9069\u7528\u6240\u6709\u7d30\u80de\u3001\u7d44\u7e54\u3001\u985e\u5668\u5b98\u6216\u751f\u7269\u6a23\u54c1\u7684\u56fa\u5b9a\u503c\u3002\r\n    <\/p>\r\n\r\n    <h3>\u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u7684\u901a\u7528\u6d41\u7a0b<\/h3>\r\n\r\n    <ol class=\"hh-seo-article__steps\">\r\n      <li>\r\n        <div>\r\n          <strong>\u9078\u64c7\u72c0\u614b\u5408\u9069\u7684\u7d30\u80de<\/strong>\r\n          <p>\u78ba\u8a8d\u7d30\u80de\u8655\u65bc\u9069\u5408\u4fdd\u5b58\u7684\u751f\u9577\u968e\u6bb5\uff0c\u4e14\u7121\u660e\u986f\u6c59\u67d3\u6216\u578b\u614b\u7570\u5e38\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u6536\u96c6\u4e26\u8a55\u4f30\u7d30\u80de<\/strong>\r\n          <p>\u9032\u884c\u7d30\u80de\u8a08\u6578\u8207\u5b58\u6d3b\u7387\u6aa2\u67e5\uff0c\u4f9d\u9700\u6c42\u8abf\u6574\u7d30\u80de\u6fc3\u5ea6\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u6e96\u5099\u51b7\u51cd\u4fdd\u5b58\u6db2<\/strong>\r\n          <p>\u4f9d\u7d30\u80de\u682a\u6307\u5f15\u9078\u64c7\u9069\u5408\u7684\u57fa\u790e\u57f9\u990a\u6db2\u3001\u86cb\u767d\u4f86\u6e90\u8207\u51b7\u51cd\u4fdd\u8b77\u5291\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u5206\u88dd\u4e26\u5b8c\u6574\u6a19\u793a<\/strong>\r\n          <p>\u6a19\u793a\u7d30\u80de\u682a\u3001\u6279\u6b21\u3001\u7e7c\u4ee3\u6b21\u6578\u3001\u7d30\u80de\u6578\u91cf\u3001\u65e5\u671f\u8207\u64cd\u4f5c\u8005\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u57f7\u884c\u53d7\u63a7\u964d\u6eab<\/strong>\r\n          <p>\u4f7f\u7528\u7d93\u9a57\u8b49\u7684\u65b9\u6cd5\u6216\u8a2d\u5099\u63a7\u5236\u964d\u6eab\u904e\u7a0b\uff0c\u907f\u514d\u76f4\u63a5\u5957\u7528\u672a\u9a57\u8b49\u7684\u56fa\u5b9a\u6642\u7a0b\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u79fb\u81f3\u9577\u671f\u5132\u5b58\u74b0\u5883<\/strong>\r\n          <p>\u5b8c\u6210\u964d\u6eab\u5f8c\uff0c\u4f9d\u7d30\u80de\u682a\u8207\u4fdd\u5b58\u9700\u6c42\u79fb\u81f3\u7b26\u5408\u898f\u7bc4\u7684\u4f4e\u6eab\u5132\u5b58\u7cfb\u7d71\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n\r\n      <li>\r\n        <div>\r\n          <strong>\u66f4\u65b0\u7d30\u80de\u5eab\u7d00\u9304<\/strong>\r\n          <p>\u767b\u9304\u51b7\u51cd\u7ba1\u4f4d\u7f6e\u3001\u6578\u91cf\u3001\u6279\u6b21\u3001\u964d\u6eab\u7a0b\u5e8f\u8207\u5f8c\u7e8c\u53d6\u7528\u7d00\u9304\u3002<\/p>\r\n        <\/div>\r\n      <\/li>\r\n    <\/ol>\r\n\r\n    <h3>\u624b\u52d5\u964d\u6eab\u3001\u7a0b\u5e8f\u964d\u6eab\u76d2\u8207\u7a0b\u5e8f\u964d\u6eab\u5100\u6709\u4f55\u5dee\u7570\uff1f<\/h3>\r\n\r\n    <p>\r\n      \u4e09\u7a2e\u65b9\u6cd5\u7684\u4e3b\u8981\u5dee\u7570\u5728\u65bc\u964d\u6eab\u63a7\u5236\u80fd\u529b\u3001\u64cd\u4f5c\u8ca0\u64d4\u3001\u7d00\u9304\u5b8c\u6574\u5ea6\u8207\u91cd\u73fe\u6027\u3002\u9078\u64c7\u6642\u61c9\u8003\u616e\u6a23\u54c1\u50f9\u503c\u3001\u6279\u6b21\u6578\u91cf\u3001\u5be6\u9a57\u983b\u7387\u53ca\u54c1\u8cea\u7ba1\u7406\u8981\u6c42\u3002\r\n    <\/p>\r\n\r\n    <div\r\n      class=\"hh-seo-article__table-wrap\"\r\n      tabindex=\"0\"\r\n      aria-label=\"\u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u964d\u6eab\u65b9\u6cd5\u6bd4\u8f03\u8868\uff0c\u53ef\u6c34\u5e73\u6372\u52d5\">\r\n\r\n      <table>\r\n        <caption>\u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u964d\u6eab\u65b9\u6cd5\u6bd4\u8f03<\/caption>\r\n\r\n        <thead>\r\n          <tr>\r\n            <th scope=\"col\">\u6bd4\u8f03\u9805\u76ee<\/th>\r\n            <th scope=\"col\">\u5206\u6bb5\u5f0f\u624b\u52d5\u964d\u6eab<\/th>\r\n            <th scope=\"col\">\u7a0b\u5e8f\u964d\u6eab\u76d2<\/th>\r\n            <th scope=\"col\">\u7a0b\u5e8f\u964d\u6eab\u5100<\/th>\r\n          <\/tr>\r\n        <\/thead>\r\n\r\n        <tbody>\r\n          <tr>\r\n            <th scope=\"row\">\u63a7\u5236\u65b9\u5f0f<\/th>\r\n            <td>\u4ee5\u4eba\u5de5\u8f49\u79fb\u81f3\u4e0d\u540c\u6eab\u5ea6\u74b0\u5883<\/td>\r\n            <td>\u5229\u7528\u5bb9\u5668\u4ecb\u8cea\u6216\u5c0e\u71b1\u7d50\u69cb\u5f62\u6210\u8fd1\u4f3c\u964d\u6eab\u901f\u7387<\/td>\r\n            <td>\u900f\u904e\u53ef\u8a2d\u5b9a\u7a0b\u5e8f\u63a7\u5236\u6a23\u54c1\u964d\u6eab\u904e\u7a0b<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u964d\u6eab\u66f2\u7dda<\/th>\r\n            <td>\u63a7\u5236\u80fd\u529b\u8f03\u6709\u9650<\/td>\r\n            <td>\u4f9d\u5bb9\u5668\u8a2d\u8a08\u8207\u4f7f\u7528\u689d\u4ef6\u800c\u5b9a<\/td>\r\n            <td>\u53ef\u4f9d\u8a2d\u5099\u80fd\u529b\u8a2d\u5b9a\u7dda\u6027\u6216\u591a\u968e\u6bb5\u7a0b\u5e8f<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u64cd\u4f5c\u8ca0\u64d4<\/th>\r\n            <td>\u9700\u8981\u4eba\u5de5\u8a08\u6642\u8207\u591a\u6b21\u79fb\u8f49<\/td>\r\n            <td>\u64cd\u4f5c\u76f8\u5c0d\u7c21\u55ae\uff0c\u4f46\u9808\u4f9d\u898f\u7bc4\u6e96\u5099\u8207\u7dad\u8b77<\/td>\r\n            <td>\u5b8c\u6210\u8a2d\u5b9a\u5f8c\u53ef\u81ea\u52d5\u57f7\u884c\uff0c\u4f46\u9700\u5efa\u7acb\u9a57\u8b49\u7a0b\u5e8f<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u8cc7\u6599\u7d00\u9304<\/th>\r\n            <td>\u4e3b\u8981\u4f9d\u8cf4\u4eba\u5de5\u7d00\u9304<\/td>\r\n            <td>\u901a\u5e38\u9700\u642d\u914d\u5176\u4ed6\u65b9\u5f0f\u8a18\u9304<\/td>\r\n            <td>\u90e8\u5206\u8a2d\u5099\u53ef\u8a18\u9304\u6eab\u5ea6\u8207\u904b\u884c\u8cc7\u6599<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u91cd\u73fe\u6027<\/th>\r\n            <td>\u8f03\u5bb9\u6613\u53d7\u5230\u64cd\u4f5c\u6642\u9593\u8207\u4eba\u54e1\u5dee\u7570\u5f71\u97ff<\/td>\r\n            <td>\u9069\u5408\u689d\u4ef6\u76f8\u5c0d\u55ae\u7d14\u3001\u6279\u6b21\u91cf\u8f03\u5c0f\u7684\u6d41\u7a0b<\/td>\r\n            <td>\u8f03\u9069\u5408\u9700\u8981\u7a0b\u5e8f\u63a7\u5236\u8207\u6279\u6b21\u8ffd\u6eaf\u7684\u61c9\u7528<\/td>\r\n          <\/tr>\r\n\r\n          <tr>\r\n            <th scope=\"row\">\u4e3b\u8981\u9650\u5236<\/th>\r\n            <td>\u4e0d\u5b9c\u76f4\u63a5\u5c07\u55ae\u4e00\u5206\u6bb5\u6642\u7a0b\u5957\u7528\u6240\u6709\u7d30\u80de<\/td>\r\n            <td>\u5be6\u969b\u964d\u6eab\u8868\u73fe\u53d7\u88dd\u8f09\u91cf\u53ca\u4f7f\u7528\u689d\u4ef6\u5f71\u97ff<\/td>\r\n            <td>\u9700\u8a55\u4f30\u8a2d\u5099\u5bb9\u91cf\u3001\u6eab\u5ea6\u7bc4\u570d\u3001\u9a57\u8b49\u8207\u7dad\u8b77\u6210\u672c<\/td>\r\n          <\/tr>\r\n        <\/tbody>\r\n      <\/table>\r\n    <\/div>\r\n\r\n    <aside class=\"hh-seo-article__note hh-seo-article__note--warning\">\r\n      <strong>\u300c\u964d\u6eab\u968e\u6bb5\u514d\u6db2\u614b\u6c2e\u300d\u4e0d\u7b49\u65bc\u300c\u9577\u671f\u4fdd\u5b58\u4e0d\u9700\u8981\u4f4e\u6eab\u5132\u5b58\u300d<\/strong>\r\n      <p>\r\n        \u7a0b\u5e8f\u964d\u6eab\u8a2d\u5099\u4e3b\u8981\u8ca0\u8cac\u63a7\u5236\u51b7\u51cd\u904e\u7a0b\u3002\u5b8c\u6210\u964d\u6eab\u5f8c\uff0c\u6a23\u54c1\u4ecd\u9808\u4f9d\u7d30\u80de\u682a\u3001\u4fdd\u5b58\u671f\u9650\u8207\u54c1\u8cea\u8981\u6c42\uff0c\u79fb\u81f3\u7b26\u5408\u898f\u7bc4\u7684\u4f4e\u6eab\u5132\u5b58\u74b0\u5883\u3002\r\n      <\/p>\r\n    <\/aside>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"post-thaw-quality\">\r\n    <div class=\"hh-seo-article__section-label\">Quality Control<\/div>\r\n\r\n    <h2>\u7d30\u80de\u89e3\u51cd\u5f8c\u61c9\u6aa2\u67e5\u54ea\u4e9b\u54c1\u8cea\u6307\u6a19\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u7d30\u80de\u89e3\u51cd\u5f8c\u4e0d\u61c9\u53ea\u78ba\u8a8d\u300c\u6709\u6c92\u6709\u5b58\u6d3b\u300d\uff0c\u9084\u9700\u89c0\u5bdf\u5b58\u6d3b\u7387\u3001\u8cbc\u9644\u6216\u6062\u5fa9\u60c5\u5f62\u3001\u7d30\u80de\u578b\u614b\u3001\u751f\u9577\u901f\u5ea6\u3001\u6c59\u67d3\u72c0\u6cc1\u8207\u7d30\u80de\u682a\u8eab\u5206\uff0c\u624d\u80fd\u5224\u65b7\u8a72\u6279\u7d30\u80de\u662f\u5426\u9069\u5408\u5f8c\u7e8c\u5be6\u9a57\u3002\r\n    <\/p>\r\n\r\n    <ul class=\"hh-seo-article__quality-list\">\r\n      <li>\r\n        <strong>\u7d30\u80de\u5b58\u6d3b\u7387<\/strong>\r\n        <span>\u6bd4\u8f03\u89e3\u51cd\u5f8c\u7684\u5b58\u6d3b\u7d30\u80de\u6578\u91cf\u53ca\u5f8c\u7e8c\u6062\u5fa9\u72c0\u614b\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u8cbc\u9644\u6216\u61f8\u6d6e\u72c0\u614b<\/strong>\r\n        <span>\u78ba\u8a8d\u7d30\u80de\u662f\u5426\u4f9d\u9810\u671f\u8cbc\u9644\u3001\u5c55\u958b\u6216\u7dad\u6301\u5408\u9069\u7684\u61f8\u6d6e\u751f\u9577\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u7d30\u80de\u578b\u614b<\/strong>\r\n        <span>\u89c0\u5bdf\u5927\u5c0f\u3001\u5f62\u72c0\u3001\u6392\u5217\u53ca\u80de\u8cea\u72c0\u614b\u662f\u5426\u7b26\u5408\u7d30\u80de\u682a\u7279\u6027\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u751f\u9577\u901f\u5ea6<\/strong>\r\n        <span>\u8a18\u9304\u6062\u5fa9\u6642\u9593\u3001\u589e\u6b96\u901f\u5ea6\u53ca\u662f\u5426\u51fa\u73fe\u7570\u5e38\u505c\u6eef\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u5fae\u751f\u7269\u6c59\u67d3<\/strong>\r\n        <span>\u6aa2\u67e5\u7d30\u83cc\u3001\u771f\u83cc\u53ca\u5176\u4ed6\u53ef\u898b\u6c59\u67d3\u8de1\u8c61\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u9ef4\u6f3f\u83cc\u6aa2\u6e2c<\/strong>\r\n        <span>\u4f9d\u5be6\u9a57\u5ba4\u54c1\u8cea\u5236\u5ea6\u5b9a\u671f\u57f7\u884c\u9ef4\u6f3f\u83cc\u6aa2\u67e5\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u7d30\u80de\u682a\u8eab\u5206<\/strong>\r\n        <span>\u9ad8\u98a8\u96aa\u6216\u91cd\u8981\u5be6\u9a57\u61c9\u4f9d\u9700\u6c42\u9032\u884c\u7d30\u80de\u682a\u9451\u5b9a\u3002<\/span>\r\n      <\/li>\r\n\r\n      <li>\r\n        <strong>\u6279\u6b21\u8207\u7e7c\u4ee3\u7d00\u9304<\/strong>\r\n        <span>\u8ffd\u8e64\u4f86\u6e90\u3001\u6279\u6b21\u3001\u7e7c\u4ee3\u6b21\u6578\u3001\u57f9\u990a\u689d\u4ef6\u8207\u64cd\u4f5c\u8005\u3002<\/span>\r\n      <\/li>\r\n    <\/ul>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"equipment-selection\">\r\n    <div class=\"hh-seo-article__section-label\">Equipment Selection<\/div>\r\n\r\n    <h2>\u5982\u4f55\u9078\u64c7\u7d30\u80de\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u8a2d\u5099\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u9078\u64c7\u7d30\u80de\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u8a2d\u5099\u6642\uff0c\u61c9\u5148\u78ba\u8a8d\u6a23\u54c1\u985e\u578b\u3001\u8655\u7406\u91cf\u3001\u6eab\u5ea6\u7bc4\u570d\u3001\u7a0b\u5e8f\u63a7\u5236\u3001\u8cc7\u6599\u7d00\u9304\u3001\u91cd\u73fe\u6027\u8207\u7dad\u8b77\u9700\u6c42\uff0c\u518d\u8a55\u4f30\u8a2d\u5099\u662f\u5426\u80fd\u6574\u5408\u81f3\u65e2\u6709\u5be6\u9a57\u5ba4\u6d41\u7a0b\u3002\r\n    <\/p>\r\n\r\n    <div class=\"hh-seo-article__selection-grid\">\r\n      <section>\r\n        <span>01<\/span>\r\n        <h3>\u6a23\u54c1\u8207\u5bb9\u91cf<\/h3>\r\n        <p>\u78ba\u8a8d\u7d30\u80de\u7a2e\u985e\u3001\u51b7\u51cd\u7ba1\u898f\u683c\u3001\u55ae\u6279\u6578\u91cf\u53ca\u672a\u4f86\u64f4\u5145\u9700\u6c42\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>02<\/span>\r\n        <h3>Temperature control<\/h3>\r\n        <p>\u78ba\u8a8d\u8a2d\u5099\u6eab\u5ea6\u7bc4\u570d\u3001\u611f\u6e2c\u65b9\u5f0f\u3001\u63a7\u5236\u7cbe\u5ea6\u53ca\u5be6\u969b\u6a23\u54c1\u6eab\u5ea6\u5dee\u7570\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>03<\/span>\r\n        <h3>\u7a0b\u5e8f\u8a2d\u5b9a<\/h3>\r\n        <p>\u8a55\u4f30\u662f\u5426\u9700\u8981\u7dda\u6027\u3001\u591a\u968e\u6bb5\u3001\u6210\u6838\u6216\u81ea\u8a02\u964d\u6eab\u7a0b\u5e8f\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>04<\/span>\r\n        <h3>\u8cc7\u6599\u8207\u8ffd\u6eaf<\/h3>\r\n        <p>\u78ba\u8a8d\u662f\u5426\u9700\u8981\u8a18\u9304\u6eab\u5ea6\u66f2\u7dda\u3001\u6279\u6b21\u8cc7\u6599\u3001\u8b66\u793a\u8207\u904b\u884c\u6b77\u7a0b\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>05<\/span>\r\n        <h3>\u64cd\u4f5c\u8207\u7dad\u8b77<\/h3>\r\n        <p>\u8a55\u4f30\u6e05\u6f54\u3001\u8017\u6750\u3001\u6821\u6b63\u3001\u7dad\u8b77\u983b\u7387\u53ca\u4eba\u54e1\u8a13\u7df4\u9700\u6c42\u3002<\/p>\r\n      <\/section>\r\n\r\n      <section>\r\n        <span>06<\/span>\r\n        <h3>\u65b9\u6cd5\u9a57\u8b49<\/h3>\r\n        <p>\u4f7f\u7528\u91cd\u8981\u6a23\u54c1\u524d\uff0c\u61c9\u4ee5\u4ee3\u8868\u6027\u7d30\u80de\u78ba\u8a8d\u964d\u6eab\u6d41\u7a0b\u8207\u89e3\u51cd\u5f8c\u54c1\u8cea\u3002<\/p>\r\n      <\/section>\r\n    <\/div>\r\n\r\n    <p>\r\n      \u5b8f\u8679\u63d0\u4f9b\u5be6\u9a57\u5ba4\u6eab\u63a7\u3001\u96e2\u5fc3\u3001\u6416\u5e8a\u3001\u6df7\u5408\u8207\u751f\u547d\u79d1\u5b78\u8a2d\u5099\uff0c\u53ef\u9032\u4e00\u6b65\u67e5\u770b\r\n      <a href=\"https:\/\/hongtronics.com\/en\/scientific-equipment-data\/\">\r\n        Grant \u79d1\u5b78\u5be6\u9a57\u5ba4\u8a2d\u5099\u8207\u6eab\u63a7\u65b9\u6848\r\n      <\/a>\u3002\r\n    <\/p>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__section\" id=\"microfluidics\">\r\n    <div class=\"hh-seo-article__section-label\">Advanced Research<\/div>\r\n\r\n    <h2>\u7d30\u80de\u57f9\u990a\u5982\u4f55\u5ef6\u4f38\u81f3\u5fae\u6d41\u63a7\u7814\u7a76\uff1f<\/h2>\r\n\r\n    <p class=\"hh-seo-article__answer\">\r\n      \u7576\u7814\u7a76\u5f9e\u4e00\u822c\u7d30\u80de\u57f9\u990a\u5ef6\u4f38\u81f3\u55ae\u7d30\u80de\u5206\u6790\u3001\u7d30\u80de\u5305\u5c01\u3001\u6db2\u6ef4\u751f\u6210\u3001\u85e5\u7269\u6e2c\u8a66\u6216\u5668\u5b98\u6676\u7247\u6642\uff0c\u53ef\u900f\u904e\u5fae\u6d41\u63a7\u6280\u8853\u7cbe\u7d30\u63a7\u5236\u5c11\u91cf\u6d41\u9ad4\u3001\u7d30\u80de\u8207\u8a66\u5291\u3002\r\n    <\/p>\r\n\r\n    <p>\r\n      \u5fae\u6d41\u63a7\u4e0d\u662f\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u6216\u51b7\u51cd\u4fdd\u5b58\u7684\u5fc5\u8981\u6b65\u9a5f\uff0c\u800c\u662f\u7d30\u80de\u5b8c\u6210\u7a69\u5b9a\u57f9\u990a\u5f8c\uff0c\u53ef\u4f9d\u7814\u7a76\u76ee\u6a19\u63a1\u7528\u7684\u9032\u968e\u5be6\u9a57\u5de5\u5177\u3002\r\n    <\/p>\r\n\r\n    <div class=\"hh-seo-article__related\">\r\n      <div>\r\n        <span>Extended Reading<\/span>\r\n        <h3>\u5fae\u6d41\u63a7\u6db2\u6ef4\u751f\u6210\u5982\u4f55\u61c9\u7528\u65bc\u7d30\u80de\u8207\u751f\u7269\u6a23\u54c1\u7814\u7a76\uff1f<\/h3>\r\n        <p>\r\n          \u9032\u4e00\u6b65\u4e86\u89e3\u6db2\u6ef4\u751f\u6210\u539f\u7406\u3001\u7cfb\u7d71\u914d\u7f6e\u3001\u6d41\u9ad4\u63a7\u5236\u53ca\u5e38\u898b\u5be6\u9a57\u554f\u984c\u3002\r\n        <\/p>\r\n      <\/div>\r\n\r\n      <a href=\"https:\/\/hongtronics.com\/en\/microfluidic-droplet-generation-guide\/\">\r\n        \u95b1\u8b80\u5fae\u6d41\u63a7\u6db2\u6ef4\u751f\u6210\u6307\u5357\r\n      <\/a>\r\n    <\/div>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__cta\" aria-labelledby=\"hh-cell-cta-title\">\r\n    <div>\r\n      <span class=\"hh-seo-article__cta-label\">Laboratory Solutions<\/span>\r\n\r\n      <h2 id=\"hh-cell-cta-title\">\r\n        \u5efa\u7acb\u66f4\u7a69\u5b9a\u3001\u53ef\u8ffd\u6eaf\u7684\u7d30\u80de\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u6d41\u7a0b\r\n      <\/h2>\r\n\r\n      <p>\r\n        \u5b8f\u8679\u53ef\u4f9d\u7d30\u80de\u57f9\u990a\u3001\u6a23\u54c1\u8655\u7406\u3001\u7a0b\u5e8f\u964d\u6eab\u53ca\u5be6\u9a57\u5ba4\u54c1\u8cea\u7ba1\u7406\u9700\u6c42\uff0c\u5354\u52a9\u8a55\u4f30\u6eab\u63a7\u3001\u96e2\u5fc3\u3001\u6df7\u5408\u3001\u6416\u5e8a\u8207\u751f\u547d\u79d1\u5b78\u8a2d\u5099\u3002\r\n      <\/p>\r\n    <\/div>\r\n\r\n    <div class=\"hh-seo-article__cta-actions\">\r\n      <a\r\n        class=\"hh-seo-article__button hh-seo-article__button--primary\"\r\n        href=\"https:\/\/hongtronics.com\/en\/scientific-equipment-data\/\">\r\n        \u67e5\u770b\u79d1\u5b78\u5be6\u9a57\u5ba4\u8a2d\u5099\r\n      <\/a>\r\n\r\n      <a\r\n        class=\"hh-seo-article__button hh-seo-article__button--secondary\"\r\n        href=\"https:\/\/hongtronics.com\/en\/cell-lab-solutions-com\/\">\r\n        \u4e86\u89e3\u7d30\u80de\u5be6\u9a57\u5ba4\u65b9\u6848\r\n      <\/a>\r\n    <\/div>\r\n  <\/section>\r\n\r\n  <section\r\n    class=\"hh-seo-article__section hh-seo-article__faq\"\r\n    id=\"frequently-asked-questions\">\r\n\r\n    <div class=\"hh-seo-article__section-label\">FAQ<\/div>\r\n\r\n    <h2>\u7d30\u80de\u57f9\u990a\u5e38\u898b\u554f\u984c<\/h2>\r\n\r\n    <div class=\"hh-seo-article__faq-list\">\r\n      <details>\r\n        <summary>\u7d30\u80de\u57f9\u990a\u7684\u4e09\u5927\u6b65\u9a5f\u662f\u4ec0\u9ebc\uff1f<\/summary>\r\n        <div>\r\n          <p>\r\n            \u7d30\u80de\u57f9\u990a\u53ef\u6982\u62ec\u70ba\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u4e09\u500b\u6838\u5fc3\u968e\u6bb5\uff0c\u5206\u5225\u8ca0\u8cac\u6062\u5fa9\u51b7\u51cd\u7d30\u80de\u3001\u7dad\u6301\u7d30\u80de\u589e\u6b96\uff0c\u4ee5\u53ca\u5efa\u7acb\u9577\u671f\u4fdd\u5b58\u7684\u7d30\u80de\u5eab\u3002\r\n          <\/p>\r\n        <\/div>\r\n      <\/details>\r\n\r\n      <details>\r\n        <summary>\u7d30\u80de\u5fa9\u7526\u70ba\u4ec0\u9ebc\u9700\u8981\u5feb\u901f\u89e3\u51cd\uff1f<\/summary>\r\n        <div>\r\n          <p>\r\n            \u5feb\u901f\u89e3\u51cd\u6709\u52a9\u65bc\u7e2e\u77ed\u7d30\u80de\u8655\u65bc\u4e0d\u5229\u6eab\u5ea6\u5340\u9593\u53ca\u51b7\u51cd\u4fdd\u5b58\u6db2\u4e2d\u7684\u6642\u9593\uff0c\u4f46\u5be6\u969b\u6c34\u6d74\u6eab\u5ea6\u3001\u89e3\u51cd\u6642\u9593\u8207\u5f8c\u7e8c\u8655\u7406\u65b9\u5f0f\u4ecd\u9808\u4f9d\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u8abf\u6574\u3002\r\n          <\/p>\r\n        <\/div>\r\n      <\/details>\r\n\r\n      <details>\r\n        <summary>\u7d30\u80de\u878d\u5408\u5ea6\u591a\u5c11\u6642\u53ef\u4ee5\u7e7c\u4ee3\u57f9\u990a\uff1f<\/summary>\r\n        <div>\r\n          <p>\r\n            \u6c92\u6709\u9069\u7528\u6240\u6709\u7d30\u80de\u682a\u7684\u56fa\u5b9a\u878d\u5408\u5ea6\uff1b\u7814\u7a76\u4eba\u54e1\u61c9\u4f9d\u7d30\u80de\u682a\u5efa\u8b70\u3001\u7d30\u80de\u578b\u614b\u3001\u751f\u9577\u901f\u5ea6\u53ca\u5f8c\u7e8c\u5be6\u9a57\u7528\u9014\u6c7a\u5b9a\u7e7c\u4ee3\u6642\u9593\u3002\r\n          <\/p>\r\n        <\/div>\r\n      <\/details>\r\n\r\n      <details>\r\n        <summary>\u7d30\u80de\u51b7\u51cd\u4fdd\u5b58\u4e00\u5b9a\u8981\u4f7f\u7528\u6bcf\u5206\u9418\u7d04 1\u00b0C \u7684\u964d\u6eab\u901f\u5ea6\u55ce\uff1f<\/summary>\r\n        <div>\r\n          <p>\r\n            \u6bcf\u5206\u9418\u7d04 1\u00b0C \u662f\u8a31\u591a\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u5e38\u898b\u7684\u8d77\u59cb\u689d\u4ef6\uff0c\u4f46\u4e0d\u662f\u6240\u6709\u7d30\u80de\u3001\u7d44\u7e54\u6216\u6a23\u54c1\u90fd\u9069\u7528\uff0c\u6700\u4f73\u964d\u6eab\u66f2\u7dda\u61c9\u4f9d\u6a23\u54c1\u7279\u6027\u8207\u9a57\u8b49\u7d50\u679c\u8a2d\u5b9a\u3002\r\n          <\/p>\r\n        <\/div>\r\n      <\/details>\r\n\r\n      <details>\r\n        <summary>\u7a0b\u5e8f\u964d\u6eab\u5100\u53ef\u4ee5\u53d6\u4ee3\u6db2\u614b\u6c2e\u5132\u5b58\u55ce\uff1f<\/summary>\r\n        <div>\r\n          <p>\r\n            \u4e0d\u4e00\u5b9a\u3002\u7a0b\u5e8f\u964d\u6eab\u5100\u4e3b\u8981\u63a7\u5236\u51b7\u51cd\u904e\u7a0b\uff0c\u5b8c\u6210\u964d\u6eab\u5f8c\u4ecd\u9808\u4f9d\u7d30\u80de\u682a\u53ca\u4fdd\u5b58\u671f\u9650\uff0c\u5c07\u6a23\u54c1\u79fb\u81f3\u7b26\u5408\u8981\u6c42\u7684\u4f4e\u6eab\u5132\u5b58\u74b0\u5883\u3002\r\n          <\/p>\r\n        <\/div>\r\n      <\/details>\r\n    <\/div>\r\n  <\/section>\r\n\r\n  <section class=\"hh-seo-article__references\" aria-labelledby=\"hh-cell-references-title\">\r\n    <h2 id=\"hh-cell-references-title\">\u53c3\u8003\u8cc7\u6599<\/h2>\r\n\r\n    <ul>\r\n      <li>\r\n        <a\r\n          href=\"https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\"\r\n          target=\"_blank\"\r\n          rel=\"noopener noreferrer\">\r\n          ATCC\uff1aAnimal Cell Culture Guide\r\n        <\/a>\r\n      <\/li>\r\n      <li>\r\n        <a\r\n          href=\"https:\/\/www.thermofisher.com\/hk\/zt\/home\/references\/gibco-cell-culture-basics.html\"\r\n          target=\"_blank\"\r\n          rel=\"noopener noreferrer\">\r\n          Gibco\uff1aCell Culture Basics\r\n        <\/a>\r\n      <\/li>\r\n      <li>\r\n        <a\r\n          href=\"https:\/\/www.thermofisher.com\/hk\/en\/home\/references\/gibco-cell-culture-basics\/cell-culture-protocols\/thawing-cells.html\"\r\n          target=\"_blank\"\r\n          rel=\"noopener noreferrer\">\r\n          Gibco\uff1aThawing Cells\r\n        <\/a>\r\n      <\/li>\r\n      <li>\r\n        <a\r\n          href=\"https:\/\/www.thermofisher.com\/hk\/en\/home\/references\/gibco-cell-culture-basics\/cell-culture-protocols\/freezing-cells.html\"\r\n          target=\"_blank\"\r\n          rel=\"noopener noreferrer\">\r\n          Gibco\uff1aFreezing Cells\r\n        <\/a>\r\n      <\/li>\r\n    <\/ul>\r\n  <\/section>\r\n\r\n<\/article>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u7d30\u80de\u57f9\u990a\u662f\u6307\u5728\u9ad4\u5916\u6a21\u64ec\u751f\u7269\u9ad4\u5167\u74b0\u5883\uff08\u7121\u83cc\u3001\u9069\u5b9c\u6eab\u5ea6\u3001\u9178\u9e7c\u5ea6\u548c\u4e00\u5b9a\u71df\u990a\u689d\u4ef6\u7b49\uff09\uff0c\u4f7f\u4e4b\u751f\u5b58\u3001\u751f\u9577\u3001\u7e41\u6b96\u4e26\u7dad\u6301\u4e3b\u8981\u7d50\u69cb\u548c\u529f\u80fd\u7684\u4e00\u7a2e\u65b9\u6cd5\u3002<\/p>\n<p>\u7d30\u80de\u57f9\u990a\u4e5f\u53eb\u7d30\u80de\u514b\u9686\u6280\u8853\uff0c\u662f\u7d30\u80de\u751f\u7269\u5b78\u7814\u7a76\u65b9\u6cd5\u4e2d\u91cd\u8981\u548c\u5e38\u7528\u6280\u8853\uff0c\u901a\u904e\u7d30\u80de\u57f9\u990a\u65e2\u53ef\u4ee5\u7372\u5f97\u5927\u91cf\u7d30\u80de\uff0c\u53c8\u53ef\u4ee5\u85c9\u6b64\u7814\u7a76\u7d30\u80de\u7684\u4fe1\u865f\u8f49\u5c0e\u3001\u7d30\u80de\u7684\u5408\u6210\u4ee3\u8b1d\u3001\u7d30\u80de\u7684\u751f\u9577\u589e\u6b96\u7b49\u3002<\/p>\n<p>\u7d30\u80de\u57f9\u990a\u7684\u5177\u9ad4\u6b65\u9a5f\u53ef\u5206\u70ba\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7d30\u80de\u50b3\u4ee3\u3001\u7d30\u80de\u51cd\u5b58\u3002<br \/>\n\u4f7f\u7528Grant CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100\u53f0\u5f0f\u8a2d\u8a08\uff0c\u5916\u5f62\u7cbe\u5de7\uff0c\u64cd\u4f5c\u7c21\u55ae\uff0c\u6709\u7121 PC\u9023\u63a5\u90fd\u53ef\u9032\u884c\uff1b\u6578\u64da\u53ef\u4ee5\u900f\u904ePC\u8edf\u9ad4\u9032\u884c\u8a18\u9304\uff0c\u51b7\u537b\u6578\u64da\u5c07\u76f4\u63a5\u986f\u793a\u5728PC\u87a2\u5e55\u4e0a\uff0cCRF-1\u7684\u51b7\u51cd\u80fd\u529b\u53ef\u9054\u96f6\u4e0b100\u2103(\u63a1\u7528\u7d30\u7ba1\u6642)\u3002<\/p>","protected":false},"author":34,"featured_media":31038,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_eb_attr":"","ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"minimal","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","ocean_post_oembed":"","ocean_post_self_hosted_media":"","ocean_post_video_embed":"","ocean_link_format":"","ocean_link_format_target":"self","ocean_quote_format":"","ocean_quote_format_link":"post","ocean_gallery_link_images":"off","ocean_gallery_id":[],"footnotes":""},"categories":[1889],"tags":[2115,2116,2113,2114],"class_list":["post-31030","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biological-technology","tag-crf-1","tag-grant","tag-2113","tag-2114","entry","has-media","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails","has-product-nav"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 5.0.2.1 - aioseo.com -->\n\t<meta name=\"description\" content=\"\u7d30\u80de\u57f9\u990a\u5305\u542b\u54ea\u4e9b\u6838\u5fc3\u6b65\u9a5f\uff1f\u672c\u6587\u6574\u7406\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u7684\u57fa\u672c\u539f\u7406\u3001\u901a\u7528\u64cd\u4f5c\u6d41\u7a0b\u53ca\u5e38\u898b\u554f\u984c\uff0c\u8aaa\u660e\u5feb\u901f\u89e3\u51cd\u3001\u7d30\u80de\u878d\u5408\u5ea6\u3001\u63a5\u7a2e\u5bc6\u5ea6\u3001\u51b7\u51cd\u4fdd\u5b58\u6db2\u8207\u53d7\u63a7\u964d\u6eab\u7684\u91cd\u8981\u6027\uff0c\u4e26\u6bd4\u8f03\u624b\u52d5\u964d\u6eab\u3001\u7a0b\u5e8f\u964d\u6eab\u76d2\u8207\u7a0b\u5e8f\u964d\u6eab\u5100\u7684\u5dee\u7570\u3002\u5be6\u969b\u6eab\u5ea6\u3001\u6642\u9593\u8207\u57f9\u990a\u689d\u4ef6\u4ecd\u61c9\u4f9d\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u53ca\u5be6\u9a57\u5ba4 SOP \u8abf\u6574\uff0c\u5354\u52a9\u7814\u7a76\u4eba\u54e1\u5efa\u7acb\u7a69\u5b9a\u3001\u53ef\u8ffd\u6eaf\u7684\u7d30\u80de\u57f9\u990a\u6d41\u7a0b\u3002\" \/>\n\t<meta name=\"robots\" content=\"max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n\t<meta name=\"author\" content=\"yuwen kan\"\/>\n\t<meta name=\"google-site-verification\" content=\"SkpHtpSLB2vAUMP2DE82RvjkPkzsxboRb7ir9XnuNmQ\" \/>\n\t<meta name=\"msvalidate.01\" content=\"75F9DB4D5EFA19B04308C9BEC35BA06B\" \/>\n\t<meta name=\"p:domain_verify\" content=\"9b5f6acf70cc242e0a343b56495c9000\" \/>\n\t<meta name=\"keywords\" content=\"\u7d30\u80de\u57f9\u990a\u6b65\u9a5f,\u7d30\u80de\u57f9\u990a,\u6c34\u6d74\u6416\u5e8a\u8a2d\u5099,crf-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100,grant,\u6280\u8853\u6587\u7ae0\" \/>\n\t<link rel=\"canonical\" href=\"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/\" \/>\n\t<meta name=\"generator\" content=\"All in One SEO (AIOSEO) 5.0.2.1\" \/>\n\t\t<script type=\"application\/ld+json\" class=\"aioseo-schema\">\n\t\t\t{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#article\",\"name\":\"\\u7d30\\u80de\\u57f9\\u990a\\u4e09\\u5927\\u6b65\\u9a5f\\u5b8c\\u6574\\u6307\\u5357\\uff1a\\u7d30\\u80de\\u5fa9\\u7526\\u3001\\u7e7c\\u4ee3\\u57f9\\u990a\\u8207\\u51b7\\u51cd\\u4fdd\\u5b58 | \\u5b8f\\u8679\\u96fb\\u5b50\\u79d1\\u6280\\u6709\\u9650\\u516c\\u53f8\",\"headline\":\"\\u7d30\\u80de\\u57f9\\u990a\\u4e09\\u5927\\u6b65\\u9a5f\\u5b8c\\u6574\\u6307\\u5357\\uff1a\\u7d30\\u80de\\u5fa9\\u7526\\u3001\\u7e7c\\u4ee3\\u57f9\\u990a\\u8207\\u51b7\\u51cd\\u4fdd\\u5b58\",\"author\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/author\\\/kyw\\\/#author\"},\"publisher\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#organization\"},\"image\":{\"@type\":\"ImageObject\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/wp-content\\\/uploads\\\/2023\\\/05\\\/700700-12.png\",\"width\":700,\"height\":700},\"datePublished\":\"2023-05-03T10:16:40+08:00\",\"dateModified\":\"2026-09-15T14:05:51+08:00\",\"inLanguage\":\"en-US\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#webpage\"},\"isPartOf\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#webpage\"},\"articleSection\":\"\\u751f\\u7269\\u79d1\\u6280, CRF-1\\u514d\\u6db2\\u6c2e\\u7a0b\\u5e8f\\u964d\\u6eab\\u5100, GRANT, \\u6280\\u8853\\u6587\\u7ae0, \\u7d30\\u80de\\u57f9\\u990a\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#breadcrumblist\",\"itemListElement\":[{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#listItem\",\"position\":1,\"name\":\"\\u4e3b\\u9801\",\"item\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/\",\"nextItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/category\\\/biological-technology\\\/#listItem\",\"name\":\"\\u751f\\u7269\\u79d1\\u6280\"}},{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/category\\\/biological-technology\\\/#listItem\",\"position\":2,\"name\":\"\\u751f\\u7269\\u79d1\\u6280\",\"item\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/category\\\/biological-technology\\\/\",\"nextItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#listItem\",\"name\":\"\\u7d30\\u80de\\u57f9\\u990a\\u4e09\\u5927\\u6b65\\u9a5f\\u5b8c\\u6574\\u6307\\u5357\\uff1a\\u7d30\\u80de\\u5fa9\\u7526\\u3001\\u7e7c\\u4ee3\\u57f9\\u990a\\u8207\\u51b7\\u51cd\\u4fdd\\u5b58\"},\"previousItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#listItem\",\"name\":\"\\u4e3b\\u9801\"}},{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#listItem\",\"position\":3,\"name\":\"\\u7d30\\u80de\\u57f9\\u990a\\u4e09\\u5927\\u6b65\\u9a5f\\u5b8c\\u6574\\u6307\\u5357\\uff1a\\u7d30\\u80de\\u5fa9\\u7526\\u3001\\u7e7c\\u4ee3\\u57f9\\u990a\\u8207\\u51b7\\u51cd\\u4fdd\\u5b58\",\"previousItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/category\\\/biological-technology\\\/#listItem\",\"name\":\"\\u751f\\u7269\\u79d1\\u6280\"}}]},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#organization\",\"name\":\"\\u5b8f\\u8679\\u96fb\\u5b50\\u79d1\\u6280\\u6709\\u9650\\u516c\\u53f8\",\"description\":\"\\u8b93\\u8eca\\u7528\\u96fb\\u5b50\\u8207\\u5de5\\u696d4.0\\u89e3\\u6c7a\\u65b9\\u6848\\u52a9\\u60a8\\u4e00\\u81c2\\u4e4b\\u529b\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/\",\"telephone\":\"+88633589488\",\"logo\":{\"@type\":\"ImageObject\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/wp-content\\\/uploads\\\/2019\\\/11\\\/\\u5b8f\\u8679_\\u753b\\u677f-12.png\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#organizationLogo\"},\"image\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#organizationLogo\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/author\\\/kyw\\\/#author\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/author\\\/kyw\\\/\",\"name\":\"yuwen kan\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#webpage\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/\",\"name\":\"\\u7d30\\u80de\\u57f9\\u990a\\u4e09\\u5927\\u6b65\\u9a5f\\u5b8c\\u6574\\u6307\\u5357\\uff1a\\u7d30\\u80de\\u5fa9\\u7526\\u3001\\u7e7c\\u4ee3\\u57f9\\u990a\\u8207\\u51b7\\u51cd\\u4fdd\\u5b58 | \\u5b8f\\u8679\\u96fb\\u5b50\\u79d1\\u6280\\u6709\\u9650\\u516c\\u53f8\",\"description\":\"\\u7d30\\u80de\\u57f9\\u990a\\u5305\\u542b\\u54ea\\u4e9b\\u6838\\u5fc3\\u6b65\\u9a5f\\uff1f\\u672c\\u6587\\u6574\\u7406\\u7d30\\u80de\\u5fa9\\u7526\\u3001\\u7e7c\\u4ee3\\u57f9\\u990a\\u8207\\u51b7\\u51cd\\u4fdd\\u5b58\\u7684\\u57fa\\u672c\\u539f\\u7406\\u3001\\u901a\\u7528\\u64cd\\u4f5c\\u6d41\\u7a0b\\u53ca\\u5e38\\u898b\\u554f\\u984c\\uff0c\\u8aaa\\u660e\\u5feb\\u901f\\u89e3\\u51cd\\u3001\\u7d30\\u80de\\u878d\\u5408\\u5ea6\\u3001\\u63a5\\u7a2e\\u5bc6\\u5ea6\\u3001\\u51b7\\u51cd\\u4fdd\\u5b58\\u6db2\\u8207\\u53d7\\u63a7\\u964d\\u6eab\\u7684\\u91cd\\u8981\\u6027\\uff0c\\u4e26\\u6bd4\\u8f03\\u624b\\u52d5\\u964d\\u6eab\\u3001\\u7a0b\\u5e8f\\u964d\\u6eab\\u76d2\\u8207\\u7a0b\\u5e8f\\u964d\\u6eab\\u5100\\u7684\\u5dee\\u7570\\u3002\\u5be6\\u969b\\u6eab\\u5ea6\\u3001\\u6642\\u9593\\u8207\\u57f9\\u990a\\u689d\\u4ef6\\u4ecd\\u61c9\\u4f9d\\u7d30\\u80de\\u682a\\u8aaa\\u660e\\u66f8\\u53ca\\u5be6\\u9a57\\u5ba4 SOP \\u8abf\\u6574\\uff0c\\u5354\\u52a9\\u7814\\u7a76\\u4eba\\u54e1\\u5efa\\u7acb\\u7a69\\u5b9a\\u3001\\u53ef\\u8ffd\\u6eaf\\u7684\\u7d30\\u80de\\u57f9\\u990a\\u6d41\\u7a0b\\u3002\",\"inLanguage\":\"en-US\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#website\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#breadcrumblist\"},\"author\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/author\\\/kyw\\\/#author\"},\"creator\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/author\\\/kyw\\\/#author\"},\"image\":{\"@type\":\"ImageObject\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/wp-content\\\/uploads\\\/2023\\\/05\\\/700700-12.png\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#mainImage\",\"width\":700,\"height\":700},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\\\/#mainImage\"},\"datePublished\":\"2023-05-03T10:16:40+08:00\",\"dateModified\":\"2026-09-15T14:05:51+08:00\"},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#website\",\"url\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/\",\"name\":\"\\u5b8f\\u8679\\u96fb\\u5b50\\u79d1\\u6280\\u6709\\u9650\\u516c\\u53f8 | \\u53f0\\u7063\\u8679\\u79d1\",\"description\":\"\\u8b93\\u8eca\\u7528\\u96fb\\u5b50\\u8207\\u5de5\\u696d4.0\\u89e3\\u6c7a\\u65b9\\u6848\\u52a9\\u60a8\\u4e00\\u81c2\\u4e4b\\u529b\",\"inLanguage\":\"en-US\",\"publisher\":{\"@id\":\"https:\\\/\\\/hongtronics.com\\\/en\\\/#organization\"}}]}\n\t\t<\/script>\n\t\t<!-- All in One SEO -->\n\n","aioseo_head_json":{"title":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58 | \u5b8f\u8679\u96fb\u5b50\u79d1\u6280\u6709\u9650\u516c\u53f8","description":"\u7d30\u80de\u57f9\u990a\u5305\u542b\u54ea\u4e9b\u6838\u5fc3\u6b65\u9a5f\uff1f\u672c\u6587\u6574\u7406\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u7684\u57fa\u672c\u539f\u7406\u3001\u901a\u7528\u64cd\u4f5c\u6d41\u7a0b\u53ca\u5e38\u898b\u554f\u984c\uff0c\u8aaa\u660e\u5feb\u901f\u89e3\u51cd\u3001\u7d30\u80de\u878d\u5408\u5ea6\u3001\u63a5\u7a2e\u5bc6\u5ea6\u3001\u51b7\u51cd\u4fdd\u5b58\u6db2\u8207\u53d7\u63a7\u964d\u6eab\u7684\u91cd\u8981\u6027\uff0c\u4e26\u6bd4\u8f03\u624b\u52d5\u964d\u6eab\u3001\u7a0b\u5e8f\u964d\u6eab\u76d2\u8207\u7a0b\u5e8f\u964d\u6eab\u5100\u7684\u5dee\u7570\u3002\u5be6\u969b\u6eab\u5ea6\u3001\u6642\u9593\u8207\u57f9\u990a\u689d\u4ef6\u4ecd\u61c9\u4f9d\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u53ca\u5be6\u9a57\u5ba4 SOP \u8abf\u6574\uff0c\u5354\u52a9\u7814\u7a76\u4eba\u54e1\u5efa\u7acb\u7a69\u5b9a\u3001\u53ef\u8ffd\u6eaf\u7684\u7d30\u80de\u57f9\u990a\u6d41\u7a0b\u3002","canonical_url":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/","robots":"max-snippet:-1, max-image-preview:large, max-video-preview:-1","keywords":"\u7d30\u80de\u57f9\u990a\u6b65\u9a5f,\u7d30\u80de\u57f9\u990a,\u6c34\u6d74\u6416\u5e8a\u8a2d\u5099,crf-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100,grant,\u6280\u8853\u6587\u7ae0","webmasterTools":{"google-site-verification":"SkpHtpSLB2vAUMP2DE82RvjkPkzsxboRb7ir9XnuNmQ","msvalidate.01":"75F9DB4D5EFA19B04308C9BEC35BA06B","p:domain_verify":"9b5f6acf70cc242e0a343b56495c9000","miscellaneous":""},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#article","name":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58 | \u5b8f\u8679\u96fb\u5b50\u79d1\u6280\u6709\u9650\u516c\u53f8","headline":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58","author":{"@id":"https:\/\/hongtronics.com\/en\/author\/kyw\/#author"},"publisher":{"@id":"https:\/\/hongtronics.com\/en\/#organization"},"image":{"@type":"ImageObject","url":"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12.png","width":700,"height":700},"datePublished":"2023-05-03T10:16:40+08:00","dateModified":"2026-09-15T14:05:51+08:00","inLanguage":"en-US","mainEntityOfPage":{"@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#webpage"},"isPartOf":{"@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#webpage"},"articleSection":"\u751f\u7269\u79d1\u6280, CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100, GRANT, \u6280\u8853\u6587\u7ae0, \u7d30\u80de\u57f9\u990a"},{"@type":"BreadcrumbList","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#breadcrumblist","itemListElement":[{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/#listItem","position":1,"name":"\u4e3b\u9801","item":"https:\/\/hongtronics.com\/en\/","nextItem":{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/category\/biological-technology\/#listItem","name":"\u751f\u7269\u79d1\u6280"}},{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/category\/biological-technology\/#listItem","position":2,"name":"\u751f\u7269\u79d1\u6280","item":"https:\/\/hongtronics.com\/en\/category\/biological-technology\/","nextItem":{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#listItem","name":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58"},"previousItem":{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/#listItem","name":"\u4e3b\u9801"}},{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#listItem","position":3,"name":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58","previousItem":{"@type":"ListItem","@id":"https:\/\/hongtronics.com\/en\/category\/biological-technology\/#listItem","name":"\u751f\u7269\u79d1\u6280"}}]},{"@type":"Organization","@id":"https:\/\/hongtronics.com\/en\/#organization","name":"\u5b8f\u8679\u96fb\u5b50\u79d1\u6280\u6709\u9650\u516c\u53f8","description":"\u8b93\u8eca\u7528\u96fb\u5b50\u8207\u5de5\u696d4.0\u89e3\u6c7a\u65b9\u6848\u52a9\u60a8\u4e00\u81c2\u4e4b\u529b","url":"https:\/\/hongtronics.com\/en\/","telephone":"+88633589488","logo":{"@type":"ImageObject","url":"https:\/\/hongtronics.com\/wp-content\/uploads\/2019\/11\/\u5b8f\u8679_\u753b\u677f-12.png","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#organizationLogo"},"image":{"@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#organizationLogo"}},{"@type":"Person","@id":"https:\/\/hongtronics.com\/en\/author\/kyw\/#author","url":"https:\/\/hongtronics.com\/en\/author\/kyw\/","name":"yuwen kan"},{"@type":"WebPage","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#webpage","url":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/","name":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58 | \u5b8f\u8679\u96fb\u5b50\u79d1\u6280\u6709\u9650\u516c\u53f8","description":"\u7d30\u80de\u57f9\u990a\u5305\u542b\u54ea\u4e9b\u6838\u5fc3\u6b65\u9a5f\uff1f\u672c\u6587\u6574\u7406\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u7684\u57fa\u672c\u539f\u7406\u3001\u901a\u7528\u64cd\u4f5c\u6d41\u7a0b\u53ca\u5e38\u898b\u554f\u984c\uff0c\u8aaa\u660e\u5feb\u901f\u89e3\u51cd\u3001\u7d30\u80de\u878d\u5408\u5ea6\u3001\u63a5\u7a2e\u5bc6\u5ea6\u3001\u51b7\u51cd\u4fdd\u5b58\u6db2\u8207\u53d7\u63a7\u964d\u6eab\u7684\u91cd\u8981\u6027\uff0c\u4e26\u6bd4\u8f03\u624b\u52d5\u964d\u6eab\u3001\u7a0b\u5e8f\u964d\u6eab\u76d2\u8207\u7a0b\u5e8f\u964d\u6eab\u5100\u7684\u5dee\u7570\u3002\u5be6\u969b\u6eab\u5ea6\u3001\u6642\u9593\u8207\u57f9\u990a\u689d\u4ef6\u4ecd\u61c9\u4f9d\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u53ca\u5be6\u9a57\u5ba4 SOP \u8abf\u6574\uff0c\u5354\u52a9\u7814\u7a76\u4eba\u54e1\u5efa\u7acb\u7a69\u5b9a\u3001\u53ef\u8ffd\u6eaf\u7684\u7d30\u80de\u57f9\u990a\u6d41\u7a0b\u3002","inLanguage":"en-US","isPartOf":{"@id":"https:\/\/hongtronics.com\/en\/#website"},"breadcrumb":{"@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#breadcrumblist"},"author":{"@id":"https:\/\/hongtronics.com\/en\/author\/kyw\/#author"},"creator":{"@id":"https:\/\/hongtronics.com\/en\/author\/kyw\/#author"},"image":{"@type":"ImageObject","url":"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12.png","@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#mainImage","width":700,"height":700},"primaryImageOfPage":{"@id":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/#mainImage"},"datePublished":"2023-05-03T10:16:40+08:00","dateModified":"2026-09-15T14:05:51+08:00"},{"@type":"WebSite","@id":"https:\/\/hongtronics.com\/en\/#website","url":"https:\/\/hongtronics.com\/en\/","name":"\u5b8f\u8679\u96fb\u5b50\u79d1\u6280\u6709\u9650\u516c\u53f8 | \u53f0\u7063\u8679\u79d1","description":"\u8b93\u8eca\u7528\u96fb\u5b50\u8207\u5de5\u696d4.0\u89e3\u6c7a\u65b9\u6848\u52a9\u60a8\u4e00\u81c2\u4e4b\u529b","inLanguage":"en-US","publisher":{"@id":"https:\/\/hongtronics.com\/en\/#organization"}}]}},"aioseo_meta_data":{"post_id":"31030","title":"#post_title #separator_sa #site_title","description":"\u7d30\u80de\u57f9\u990a\u5305\u542b\u54ea\u4e9b\u6838\u5fc3\u6b65\u9a5f\uff1f\u672c\u6587\u6574\u7406\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\u7684\u57fa\u672c\u539f\u7406\u3001\u901a\u7528\u64cd\u4f5c\u6d41\u7a0b\u53ca\u5e38\u898b\u554f\u984c\uff0c\u8aaa\u660e\u5feb\u901f\u89e3\u51cd\u3001\u7d30\u80de\u878d\u5408\u5ea6\u3001\u63a5\u7a2e\u5bc6\u5ea6\u3001\u51b7\u51cd\u4fdd\u5b58\u6db2\u8207\u53d7\u63a7\u964d\u6eab\u7684\u91cd\u8981\u6027\uff0c\u4e26\u6bd4\u8f03\u624b\u52d5\u964d\u6eab\u3001\u7a0b\u5e8f\u964d\u6eab\u76d2\u8207\u7a0b\u5e8f\u964d\u6eab\u5100\u7684\u5dee\u7570\u3002\u5be6\u969b\u6eab\u5ea6\u3001\u6642\u9593\u8207\u57f9\u990a\u689d\u4ef6\u4ecd\u61c9\u4f9d\u7d30\u80de\u682a\u8aaa\u660e\u66f8\u53ca\u5be6\u9a57\u5ba4 SOP \u8abf\u6574\uff0c\u5354\u52a9\u7814\u7a76\u4eba\u54e1\u5efa\u7acb\u7a69\u5b9a\u3001\u53ef\u8ffd\u6eaf\u7684\u7d30\u80de\u57f9\u990a\u6d41\u7a0b\u3002","keywords":[{"label":"\u7d30\u80de\u57f9\u990a\u6b65\u9a5f","value":"\u7d30\u80de\u57f9\u990a\u6b65\u9a5f"},{"label":"\u7d30\u80de\u57f9\u990a","value":"\u7d30\u80de\u57f9\u990a"},{"label":"\u6c34\u6d74\u6416\u5e8a\u8a2d\u5099","value":"\u6c34\u6d74\u6416\u5e8a\u8a2d\u5099"},{"label":"CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100","value":"CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100"},{"label":"GRANT","value":"GRANT"}],"keyphrases":{"focus":{"keyphrase":"","score":0,"analysis":{"keyphraseInTitle":{"score":0,"maxScore":9,"error":1}}},"additional":[]},"primary_term":null,"canonical_url":null,"og_title":"#post_title #separator_sa #site_title","og_description":"\u7d30\u80de\u57f9\u990a\u662f\u6307\u5728\u9ad4\u5916\u6a21\u64ec\u751f\u7269\u9ad4\u5167\u74b0\u5883\uff0c\u4f7f\u4e4b\u751f\u5b58\u3001\u751f\u9577\u3001\u7e41\u6b96\u4e26\u7dad\u6301\u4e3b\u8981\u7d50\u69cb\u548c\u529f\u80fd\u7684\u4e00\u7a2e\u65b9\u6cd5\u3002\u7d30\u80de\u57f9\u990a\u7684\u5177\u9ad4\u6b65\u9a5f\u53ef\u5206\u70ba\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7d30\u80de\u50b3\u4ee3\u3001\u7d30\u80de\u51cd\u5b58\u3002\u4f7f\u7528Grant CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100\u53f0\u5f0f\u8a2d\u8a08\uff0c\u6578\u64da\u53ef\u4ee5\u900f\u904ePC\u8edf\u9ad4\u9032\u884c\u8a18\u9304\uff0c\u51b7\u537b\u6578\u64da\u5c07\u76f4\u63a5\u986f\u793a\u5728PC\u87a2\u5e55\u4e0a\uff0cCRF-1\u7684\u51b7\u51cd\u80fd\u529b\u53ef\u9054\u96f6\u4e0b100\u2103\u3002","og_object_type":"default","og_image_type":"featured","og_image_url":"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12.png","og_image_width":"700","og_image_height":"700","og_image_custom_url":null,"og_image_custom_fields":null,"og_video":"","og_custom_url":null,"og_article_section":null,"og_article_tags":null,"twitter_use_og":false,"twitter_card":"default","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":[],"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"Article","isEnabled":true},"graphs":[]},"schema_type":"default","schema_type_options":null,"pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":"-1","robots_max_videopreview":"-1","robots_max_imagepreview":"large","priority":null,"frequency":"default","local_seo":null,"breadcrumb_settings":null,"limit_modified_date":false,"ai":{"faqs":[],"keyPoints":[],"schemas":[],"titles":[],"descriptions":[],"socialPosts":{"email":{"subject":"","preview":"","content":""},"linkedin":[],"twitter":[],"facebook":[],"instagram":[]}},"created":"2023-05-03 01:10:15","updated":"2026-09-15 06:05:51","seo_analyzer_scan_date":null,"focus_keyword":null,"additional_keywords":null,"truseo_locale":null},"aioseo_breadcrumb":"<div class=\"aioseo-breadcrumbs\"><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/hongtronics.com\/en\/\" title=\"\u4e3b\u9801\">\u4e3b\u9801<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">\u00bb<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/hongtronics.com\/en\/category\/biological-technology\/\" title=\"\u751f\u7269\u79d1\u6280\">\u751f\u7269\u79d1\u6280<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">\u00bb<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\t\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"\u4e3b\u9801","link":"https:\/\/hongtronics.com\/en\/"},{"label":"\u751f\u7269\u79d1\u6280","link":"https:\/\/hongtronics.com\/en\/category\/biological-technology\/"},{"label":"\u7d30\u80de\u57f9\u990a\u4e09\u5927\u6b65\u9a5f\u5b8c\u6574\u6307\u5357\uff1a\u7d30\u80de\u5fa9\u7526\u3001\u7e7c\u4ee3\u57f9\u990a\u8207\u51b7\u51cd\u4fdd\u5b58","link":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/"}],"_links":{"self":[{"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/posts\/31030","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/users\/34"}],"replies":[{"embeddable":true,"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/comments?post=31030"}],"version-history":[{"count":0,"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/posts\/31030\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/media\/31038"}],"wp:attachment":[{"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/media?parent=31030"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/categories?post=31030"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hongtronics.com\/en\/wp-json\/wp\/v2\/tags?post=31030"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}