{"id":31030,"date":"2023-05-03T10:16:40","date_gmt":"2023-05-03T02:16:40","guid":{"rendered":"https:\/\/hongtronics.com\/?p=31030"},"modified":"2023-05-03T10:16:41","modified_gmt":"2023-05-03T02:16:41","slug":"article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation","status":"publish","type":"post","link":"https:\/\/hongtronics.com\/en\/article-biotech-the-three-major-steps-of-cell-culture-recovery-subculture-cryopreservation\/","title":{"rendered":"5\u5206\u9418\u5e36\u60a8\u4e86\u89e3\u7d30\u80de\u57f9\u990a\u7684\u4e09\u5927\u6b65\u9a5f\u2014\u5fa9\u7526\u3001\u50b3\u4ee3\u3001\u51cd\u5b58"},"content":{"rendered":"<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12.png\" alt=\"\u7d30\u80de\u57f9\u990a\u7684\u4e09\u5927\u6b65\u9a5f\u2014\u5fa9\u7526\u3001\u50b3\u4ee3\u3001\u51cd\u5b58\" class=\"wp-image-31038\" srcset=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12.png 700w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12-300x300.png 300w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12-100x100.png 100w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12-600x600.png 600w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/700700-12-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>\u7d30\u80de\u57f9\u990a<\/strong>\u662f\u6307\u5728\u9ad4\u5916\u6a21\u64ec\u751f\u7269\u9ad4\u5167\u74b0\u5883\uff08\u7121\u83cc\u3001\u9069\u5b9c\u6eab\u5ea6\u3001\u9178\u9e7c\u5ea6\u548c\u4e00\u5b9a\u71df\u990a\u689d\u4ef6\u7b49\uff09\uff0c\u4f7f\u4e4b\u751f\u5b58\u3001\u751f\u9577\u3001\u7e41\u6b96\u4e26\u7dad\u6301\u4e3b\u8981\u7d50\u69cb\u548c\u529f\u80fd\u7684\u4e00\u7a2e\u65b9\u6cd5\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u7d30\u80de\u57f9\u990a\u4e5f\u53eb\u7d30\u80de\u514b\u9686\u6280\u8853\uff0c\u662f\u7d30\u80de\u751f\u7269\u5b78\u7814\u7a76\u65b9\u6cd5\u4e2d\u91cd\u8981\u548c\u5e38\u7528\u6280\u8853\uff0c\u901a\u904e\u7d30\u80de\u57f9\u990a\u65e2\u53ef\u4ee5\u7372\u5f97\u5927\u91cf\u7d30\u80de\uff0c\u53c8\u53ef\u4ee5\u85c9\u6b64\u7814\u7a76\u7d30\u80de\u7684\u4fe1\u865f\u8f49\u5c0e\u3001\u7d30\u80de\u7684\u5408\u6210\u4ee3\u8b1d\u3001\u7d30\u80de\u7684\u751f\u9577\u589e\u6b96\u7b49\u3002<\/p>\n\n\n\n<p class=\"has-text-color has-medium-font-size wp-block-paragraph\" style=\"color:#3d3b3a\">\u7d30\u80de\u57f9\u990a\u7684\u5177\u9ad4\u6b65\u9a5f\u53ef\u5206\u70ba\uff1a<strong>\u7d30\u80de\u5fa9\u7526\u3001\u7d30\u80de\u50b3\u4ee3\u3001\u7d30\u80de\u51cd\u5b58<\/strong><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"has-text-color wp-block-heading\" style=\"color:#52c198;font-size:30px\">\u4e00\u3001\u7d30\u80de\u5fa9\u7526<\/h2>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>1.\u7d30\u80de\u5fa9\u7526\u7684\u539f\u5247\uff1a\u5feb\u901f\u878d\u5316<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u5728\u5fa9\u7526\u7d30\u80de\u6642\u5fc5\u9808\u9075\u5faa\u5feb\u901f\u878d\u5316\u7684\u539f\u5247\uff0c\u5373\u5fc5\u9808\u5c07\u51cd\u5b58\u5728 -196\u2103\u6db2\u6c2e\u4e2d\u7684\u7d30\u80de\u5feb\u901f\u878d\u5316\u81f3 37 \u2103\uff0c\u56e0\u70ba\u9019\u6a23\u53ef\u4f7f\u51cd\u5b58\u6642\u7d30\u80de\u5916\u7684\u51b0\u6676\u8fc5\u901f\u878d\u5316\uff0c\u907f\u514d\u51b0\u6676\u7de9\u6162\u878d\u5316\u6642\u9032\u5165\u7d30\u80de\u5f62\u6210\u518d\u7d50\u6676\uff0c\u5c0d\u7d30\u80de\u9020\u6210\u640d\u5bb3\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>2.\u7d30\u80de\u5fa9\u7526\u64cd\u4f5c\u904e\u7a0b<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"416\" src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1024x416.png\" alt=\"\" class=\"wp-image-31031\" srcset=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1024x416.png 1024w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-300x122.png 300w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-600x243.png 600w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-768x312.png 768w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1536x623.png 1536w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image.png 1688w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">\u7d30\u80de\u5fa9\u7526\u904e\u7a0b<\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"has-text-color wp-block-heading\" style=\"color:#52c198;font-size:30px\">\u4e8c\u3001\u7d30\u80de\u50b3\u4ee3<\/h2>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">1.<strong>\u7d30\u80de\u50b3\u4ee3\u4e2d\u9047\u5230\u7684\u554f\u984c\u53ca\u89e3\u6c7a\u65b9\u6848\uff1a<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>\uff081\uff09\u50b3\u4ee3\u5bc6\u5ea6\u904e\u9ad8<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u4e00\u65e6\u7d30\u80de\u990a\u592a\u4e45\u81f3\u878d\u5408\u5ea6\u904e\u9ad8\uff08&gt;90%\uff09\u624d\u50b3\u4ee3\uff0c\u5bb9\u6613\u4f7f\u7d30\u80de\u7522\u751f\u8001\u5316\u73fe\u8c61\uff0c\u751a\u81f3\u662f\u5806\u758a\uff0c\u518d\u6d88\u5316\u7d30\u80de\u6642\u4e0d\u6613\u5439\u6253\u6210\u55ae\u9846\u7d30\u80de\uff0c\u5373\u4fbf\u518d\u91cd\u65b0\u92ea\u76e4\u50b3\u4ee3\uff0c\u7d30\u80de\u4e5f\u7121\u6cd5\u56de\u5230\u539f\u672c\u7684\u7279\u6027\u4e86\u3002\uff08\u5982\uff1a\u55ae\u5c64\u7d30\u80de\uff0c\u6c92\u9577\u6eff\u5c31\u767c\u751f\u5806\u758a\u73fe\u8c61\uff09<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>Solution:<\/strong>\u76e1\u91cf\u907f\u514d\u878d\u5408\u5ea6\u904e\u9ad8\uff0c\u93e1\u4e0b\u89c0\u5bdf\u878d\u5408\u5ea6\u7d04\u9054\u5230 80%\u5373\u53ef\u50b3\u4ee3\u5206\u76e4\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>\uff082\uff09\u50b3\u4ee3\u5bc6\u5ea6\u4f4e<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u54fa\u4e73\u52d5\u7269\u8cbc\u58c1\u57f9\u990a\u7d30\u80de\uff0c\u82e5\u7d30\u80de\u57f9\u990a\u5230 80-90%\u5bc6\u5ea6\u9700\u8981\u958b\u59cb\u50b3\u4ee3\uff0c\u5728\u50b3\u4ee3\u63a5\u7a2e\u7d30\u80de\u5bc6\u5ea6\u904e\u4f4e\uff0c\u7d30\u80de\u9593\u8ddd\u96e2\u592a\u9060\uff0c\u5c31\u7121\u6cd5\u5728\u7d30\u80de\u9593\u7522\u751f\u9ecf\u9644\u53ca\u901a\u4fe1\u4f5c\u7528\uff0c\u5e6b\u52a9\u8a0a\u865f\u50b3\u5c0e\u4e26\u6d3b\u5316\u7d30\u80de\uff1b\u7e3d\u9ad4\u7d30\u80de\u91cf\u4e0d\u8db3\uff0c\u5206\u6ccc\u7684\u751f\u9577\u56e0\u5b50\u6fc3\u5ea6\u6703\u4e0d\u8db3\u4ee5\u7522\u751f\u5229\u65bc\u751f\u9577\u7684\u5fae\u74b0\u5883\uff0c\u4ee5\u4e0a\u7686\u6703\u9020\u6210\u589e\u6b96\u901f\u5ea6\u9072\u7de9\u6216\u7d30\u80de\u6b7b\u4ea1\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>Solution:<\/strong>\u7d30\u80de\u50b3\u4ee3\u6642\uff0c\u8981\u9032\u884c\u6e96\u78ba\u7684\u7d30\u80de\u8a08\u6578\uff0c\u78ba\u4fdd\u92ea\u76e4\u7684\u5bc6\u5ea6\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">2.<strong>\u7d30\u80de\u50b3\u4ee3\u64cd\u4f5c\u904e\u7a0b\uff1a<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"972\" src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1-1024x972.png\" alt=\"\" class=\"wp-image-31032\" srcset=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1-1024x972.png 1024w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1-300x285.png 300w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1-600x569.png 600w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1-768x729.png 768w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-1.png 1152w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><strong>\u7d30\u80de\u50b3\u4ee3\u64cd\u4f5c\u904e\u7a0b<\/strong><\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"has-text-color wp-block-heading\" style=\"color:#52c198;font-size:30px\">\u4e09\u3001\u7d30\u80de\u51cd\u5b58<\/h2>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">1.<strong>\u7d30\u80de\u51cd\u5b58\u7684\u57fa\u672c\u539f\u7406\uff1a\u6162\u51cd<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>(1) \u624b\u52d5\u964d\u6eab<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u5c07\u7d30\u80de\u4f9d\u5e8f\u653e\u57284\u2103\uff0830\u5206\u9418\uff09\u3001-20\u2103\uff081\u5c0f\u6642\uff09\u3001-80\u2103\uff08\u904e\u591c\uff09\u5f8c\u79fb\u81f3\u6db2\u6c2e\u4e2d\u4fdd\u5b58\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>(2) \u7a0b\u5e8f\u964d\u6eab\u76d2<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u662f\u4e00\u822c\u6700\u5ee3\u6cdb\u4f7f\u7528\u7684\u964d\u6eab\u6cd5\uff0c\u70ba\u4e00\u7a2e\u7279\u88fd\u51b7\u51cd\u5bb9\u5668\uff08\u586b\u5145\u7570\u4e19\u9187\u6216\u4f9d\u9760\u7279\u88fd\u91d1\u5c6c\u5c0e\u71b1\uff09\uff0c\u4f7f\u7d30\u80de\u4ee5\u6bcf\u5206\u9418\u7d04 -1\u2103\u7684\u901f\u5ea6\u964d\u81f3 -80\u2103\uff08\u904e\u591c\uff09\uff0c\u7136\u5f8c\u79fb\u81f3\u6db2\u6c2e\u4e2d\u4fdd\u5b58\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>(3) \u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100<\/strong><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">\u4e00\u7a2e\u65b0\u578b\u81ea\u52d5\u5316\u7a0b\u5e8f\u964d\u6eab\u65b9\u6cd5\uff0c\u901a\u904e\u5fae\u8655\u7406\u5668\u7cbe\u78ba\u63a7\u88fd\u51b7\u537b\u901f\u7387\u548c\u6837\u54c1\u6eab\u5ea6\uff0c\u80fd\u5920\u63a7\u88fd\u6a23\u54c1\u7dda\u6027\/\u975e\u7dda\u6027\u964d\u6eab\uff0c\u80fd\u5920\u6709\u6548\u6e1b\u5c11\u51cd\u878d\u904e\u7a0b\u5c0d\u65bc\u7d30\u80de\u6216\u7d44\u7e54\u7684\u50b7\u5bb3\uff0c\u91cd\u73fe\u6027\u597d\u3001\u964d\u6eab\u904e\u7a0b\u53ef\u63a7\u3001\u6210\u672c\u4f4e\u3002\uff08\u4f8b\u5982\u9ede\u6210\u751f\u7269CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100\uff09<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">2.<strong>\u7d30\u80de\u51cd\u5b58\u64cd\u4f5c\u904e\u7a0b\uff1a<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"603\" src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3-1024x603.png\" alt=\"\" class=\"wp-image-31034\" srcset=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3-1024x603.png 1024w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3-300x177.png 300w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3-600x353.png 600w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3-768x452.png 768w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3-1536x904.png 1536w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-3.png 1600w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><strong>\u7d30\u80de\u51cd\u5b58\u64cd\u4f5c\u904e\u7a0b<\/strong><\/figcaption><\/figure>\n\n\n\n<div style=\"height:39px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"has-text-align-center has-background wp-block-heading\" style=\"background-color:#b6f5ea;font-size:30px\">Product Recommendation<\/h2>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><a href=\"https:\/\/hongtronics.com\/en\/product\/crf-1-controlled-freezer\/\" target=\"_blank\" rel=\"noreferrer noopener\"><img loading=\"lazy\" decoding=\"async\" width=\"307\" height=\"406\" src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-4.png\" alt=\"\" class=\"wp-image-31035\" srcset=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-4.png 307w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-4-300x397.png 300w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-4-227x300.png 227w\" sizes=\"(max-width: 307px) 100vw, 307px\" \/><\/a><figcaption class=\"wp-element-caption\">CRF-1<strong>\u514d<\/strong>\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"603\" height=\"323\" src=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-5.png\" alt=\"\" class=\"wp-image-31036\" srcset=\"https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-5.png 603w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-5-300x161.png 300w, https:\/\/hongtronics.com\/wp-content\/uploads\/2023\/05\/image-5-600x321.png 600w\" sizes=\"(max-width: 603px) 100vw, 603px\" \/><figcaption class=\"wp-element-caption\">\u964d\u6eab\u5100\u9802\u84cb\u6253\u958b\u793a\u610f\u5716<\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">Grant CRF-1\u514d\u6db2\u6c2e\u7a0b\u5e8f\u964d\u6eab\u5100\u53f0\u5f0f\u8a2d\u8a08\uff0c\u5916\u5f62\u7cbe\u5de7\uff0c\u64cd\u4f5c\u7c21\u55ae\uff0c<strong>\u6709\u7121 PC\u9023\u63a5\u90fd\u53ef\u9032\u884c<\/strong>\uff1b\u6578\u64da\u53ef\u4ee5\u900f\u904ePC\u8edf\u9ad4\u9032\u884c\u8a18\u9304\uff0c\u51b7\u537b\u6578\u64da\u5c07\u76f4\u63a5\u986f\u793a\u5728PC\u87a2\u5e55\u4e0a\uff0cCRF-1\u7684\u51b7\u51cd\u80fd\u529b\u53ef\u9054\u96f6\u4e0b100\u2103(\u63a1\u7528\u7d30\u7ba1\u6642)\u3002 CRF-1<strong>\u7cbe\u6e96\u63a7\u5236\u964d\u6eab\u901f\u7387<\/strong>\uff0c\u5f9e\u800c\u78ba\u4fdd\u6574\u500b\u51b7\u51cd\u904e\u7a0b\u4e2d\u6eab\u5ea6\u7684\u7cbe\u78ba\u6027\u548c\u91cd\u73fe\u6027\uff0c\u5c24\u5176\u662f\u5728\u91cd\u8981\u7684\u6210\u6838\/\u64ad\u7a2e\u968e\u6bb5\uff0c\u9019\u5c31<strong>\u78ba\u4fdd\u4e86\u7d30\u80de\u89e3\u51cd\u5f8c\u7684\u6700\u4f73\u6062\u5fa9<\/strong>\u3002<\/p>\n\n\n\n<p class=\"has-medium-font-size 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