{"id":22445,"date":"2022-08-19T09:28:38","date_gmt":"2022-08-19T01:28:38","guid":{"rendered":"https:\/\/hongtronics.com\/?p=22445"},"modified":"2024-07-12T17:12:47","modified_gmt":"2024-07-12T09:12:47","slug":"celesta-use-craft-id-technology","status":"publish","type":"post","link":"https:\/\/hongtronics.com\/en\/celesta-use-craft-id-technology\/","title":{"rendered":"\u3010\u6848\u4f8b\u5206\u4eab\u3011CELESTA\u5149\u6e90\u61c9\u7528\u65bcCraft-ID\u6280\u8853\u7684\u5171\u7126\u8ddd\u6210\u50cf"},"content":{"rendered":"
\n
01 \u4ec0\u9ebc\u662fCRISPR?<\/a><\/div>\n<\/div>\n\n\n\n

\u57fa\u56e0\u651c\u5e36\u8457\u751f\u7269\u9ad4\u7684\u907a\u50b3\u4fe1\u606f\uff0c\u662f\u5132\u5b58\u8457\u751f\u547d\u7684\u7a2e\u65cf\u3001\u8840\u578b\u3001\u5b55\u80b2\u3001\u751f\u9577\u3001\u51cb\u4ea1\u7b49\u904e\u7a0b\u7684\u4e00\u5957\u7cbe\u78ba\u5bc6\u78bc\uff0c\u6c7a\u5b9a\u751f\u7269\u9ad4\u7684\u6027\u72c0\u3002<\/p>\n\n\n\n

\u96a8\u8457\u79d1\u5b78\u7684\u6df1\u5165\u7814\u7a76\uff0c\u67d0\u4e9b\u5834\u666f\u6211\u5011\u9700\u8981\u4eba\u70ba\u6539\u8b8a\u9019\u4e9b\u5bc6\u78bc\uff0c\u8abf\u63a7\u751f\u7269\u9ad4\u7684\u5404\u7a2e\u6027\u72c0\uff0c\u4f8b\u5982\u6027\u5225\u3001\u6bdb\u8272\u3001\u819a\u8272\u7b49(\u7576\u7136\u6027\u72c0\u4e5f\u6703\u53d7\u5230\u74b0\u5883\u5f71\u97ff)\uff0c\u9019\u7a2e\u884c\u70ba\u88ab\u7a31\u70ba\u57fa\u56e0\u7de8\u8f2f\u3002\u7136\u800c\u57fa\u56e0\u7de8\u8f2f\u4e26\u975e\u6613\u4e8b\uff0c\u539f\u56e0\u4e4b\u4e00\u4fbf\u662f\u57fa\u56e0\u773e\u591a\uff0c\u7d44\u6210\u7c21\u55ae\u751f\u547d\u6700\u5c11\u8981 265 \u5230 350\u500b\u57fa\u56e0\u3002<\/p>\n\n\n\n

\u9019\u4e9b\u57fa\u56e0\u6c92\u6709\u6a19\u7c64\uff0c\u7121\u5f9e\u77e5\u9053\u54ea\u6bb5\u57fa\u56e0\u5c0d\u61c9\u4ec0\u9ebc\u73fe\u72c0\uff0c\u800c\u9019\u4e9b\u57fa\u56e0\u5b58\u5728\u65bc\u5c0f\u7684DNA\u4e2d\uff0c\u5c3a\u5ea6\u5728\u5948\u7c73\u7b49\u7d1a\u3002\u5c0f\u800c\u7e41\u96dc\uff0c\u4f7f\u5f97\u57fa\u56e0\u7de8\u8f2f\u5c31\u50cf\u662f\u5728\u4e00\u5718\u96dc\u4e82\u4e2d\uff0c\u627e\u5230\u60f3\u7de8\u8f2f\u7684\u4e00\u6bb5\u4f86\u9032\u884c\u5fae\u578b\u624b\u8853\uff0c\u96e3\u5ea6\u4e0d\u8a00\u800c\u55bb\u3002<\/p>\n\n\n\n

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\"\"<\/figure>\n<\/figure>\n\n\n\n
<\/div>\n\n\n\n

\u800c\u57281987\u5e74\u88ab\u79d1\u5b78\u5bb6\u5728\u5927\u8178\u687f\u83cc\u4e2d\u767c\u73fe\u7684\u4e00\u6bb5”\u7121\u6cd5\u78ba\u5b9a\u529f\u80fd”\u7684DNA\u6210\u70ba\u4e86\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u7684\u4e00\u5927\u5951\u6a5f\uff0c\u9019\u6bb5DNA\u5f8c\u671f\u88ab\u7a31\u70baCRISPR\u3002<\/p>\n\n\n\n

CRISPR\u662f\u751f\u547d\u6f14\u5316\u6b77\u53f2\u4e0a\uff0c\u7d30\u83cc\u548c\u75c5\u6bd2\u9032\u884c\u9b25\u722d\u7522\u751f\u7684\u514d\u75ab\u6b66\u5668\uff0c\u7c21\u55ae\u8aaa\u5c31\u662f\u75c5\u6bd2\u80fd\u628a\u81ea\u5df1\u7684\u57fa\u56e0\u6574\u5408\u5230\u7d30\u83cc\uff0c\u5229\u7528\u7d30\u83cc\u7684\u7d30\u80de\u5de5\u5177\u70ba\u81ea\u5df1\u7684\u57fa\u56e0\u8907\u88fd\u670d\u52d9\uff0c\u7d30\u83cc\u70ba\u4e86\u5c07\u75c5\u6bd2\u7684\u5916\u4f86\u5165\u4fb5\u57fa\u56e0\u6e05\u9664\uff0c\u9032\u5316\u51faCRISPR-Cas9\u7cfb\u7d71\uff0c\u5229\u7528\u9019\u500b\u9019\u500b\u7cfb\u7d71\uff0c\u7d30\u83cc\u53ef\u4ee5\u4e0d\u52d5\u8072\u8272\u5730\u628a\u57fa\u56e0\u75c5\u6bd2\u5f9e\u81ea\u5df1\u7684\u57fa\u56e0\u4e0a\u5243\u9664\uff0c\u9019\u662f\u7d30\u83cc\u7279\u6709\u7684\u514d\u75ab\u7cfb\u7d71\uff0c\u662f\u53e4\u83cc\u548c\u7d30\u83cc\u62b5\u6297\u75c5\u6bd2\u7b49\u5916\u4f86\u907a\u50b3\u7269\u8cea\u5165\u4fb5\u7684\u4e00\u7a2e\u7372\u5f97\u6027\u514d\u75ab\u7cfb\u7d71\u3002<\/p>\n\n\n\n

\u5fae\u751f\u7269\u5b78\u5bb6\u638c\u63e1\u7d30\u83cc\u64c1\u6709\u591a\u7a2e\u5207\u9664\u5916\u4f86\u75c5\u6bd2\u57fa\u56e0\u514d\u75ab\u529f\u80fd\uff0c\u5176\u4e2d\u6bd4\u8f03\u5178\u578b\u7684\u6a21\u5f0f\u662f\u4f9d\u9760\u4e00\u500b\u8907\u5408\u7269\uff0c\u8a72\u8907\u5408\u7269\u80fd\u5728\u4e00\u6bb5RNA\u6307\u5c0e\u4e0b\uff0c\u5b9a\u5411\u5c0b\u627e\u76ee\u6a19DNA\u5e8f\u5217\uff0c\u7136\u5f8c\u5c07\u8a72\u5e8f\u5217\u9032\u884c\u5243\u9664\u3002\u8a31\u591a\u7d30\u83cc\u514d\u75ab\u8907\u5408\u7269\u90fd\u76f8\u5c0d\u8907\u96dc\uff0c\u5176\u4e2d\u79d1\u5b78\u5bb6\u638c\u63e1\u5c0d\u4e00\u7a2e\u86cb\u767dCas\u7684\u64cd\u4f5c\u6280\u8853\uff0c\u4e26\u5148\u5f8c\u5c0d\u591a\u7a2e\u76ee\u6a19\u7d30\u80deDNA\u9032\u884c\u5243\u9664\u3002<\/p>\n\n\n\n

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\"\"<\/figure>\n<\/figure>\n\n\n\n
<\/div>\n\n\n\n

\u7c21\u55ae\u4f86\u8aaa\uff0cCRISPR\u6280\u8853\u662f\u4e00\u7a2e\u57fa\u56e0\u7de8\u8f2f(\u57fa\u56e0\u5254\u9664)\u7684\u6280\u8853<\/strong>\u3002\u9019\u7a2e\u6280\u8853\u7121\u8996\u57fa\u56e0\u5e36\u4f86\u7684\u6311\u6230\uff0c\u5b83\u7684\u5f37\u5927\u4e4b\u8655\u5728\u65bc\u80fd\u5920\u5be6\u73fe\u7cbe\u78ba\u7684\u57fa\u56e0\u7de8\u8f2f\uff0c\u9054\u5230\u6307\u5230\u54ea\u6253\u5230\u54ea\u7684\u7a0b\u5ea6\uff0c\u5be6\u73fe\u66f4\u9ad8\u6548\u7387\u7684\u57fa\u56e0\u5243\u9664\u3002<\/p>\n\n\n\n

\u904e\u53bb\u5341\u5e74\u88e1\uff0c\u5c0d\u65bcCRISPR\u6280\u8853\u7684\u7814\u7a76\u6709\u7206\u70b8\u6027\u6210\u9577\uff0c\u9019\u4e9b\u7814\u7a76\u5e36\u4f86\u66f4\u5275\u65b0\u7684\u61c9\u7528\uff0c\u6bd4\u5982\u8aaaCRISPR\u6280\u8853\u5c0dDNA\u5948\u7c73\u7d50\u69cb\u9032\u884c\u5de5\u7a0b\u8a2d\u8a08\u6216\u662f\u901a\u904e\u57fa\u56e0\u7de8\u8f2f\u4f86\u6cbb\u7642\u907a\u50b3\u75be\u75c5\uff0c\u9019\u4e9b\u7a81\u7834\u6027\u7814\u7a76\u9769\u65b0\u4e86\u50b3\u7d71\u6280\u8853\u57fa\u56e0\u7de8\u8f2f\u3002<\/p>\n\n\n\n

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02 CRISPR\u5149\u6e90\u7528\u65bcCRaft-ID\u6210\u50cf<\/a><\/div>\n<\/div>\n\n\n\n

\u6df7\u548c\u57fa\u56e0\u5243\u9664\u7be9\u9078\u5728\u529f\u80fd\u57fa\u56e0\u7d44\u5b78\u754c\u5ee3\u6cdb\u61c9\u7528\u65bc\u9451\u5b9a\u8207\u7d30\u80de\u8868\u578b\u76f8\u95dc\u7684\u57fa\u56e0\uff0c\u800c\u57fa\u56e0\u5243\u9664\u65b9\u6cd5\u4e2d\u6700\u5178\u578b\u7684\u5247\u70baCRISPR\u65b9\u6cd5\uff0c\u4f46\u662f\u985e\u7be9\u9078\u65b9\u6cd5\u5927\u591a\u63a1\u7528\u6bd4\u8f03\u7c97\u66e0\u7684\u7be9\u9078\u6307\u6a19\uff0c\u5982\u751f\u9577\u901f\u7387\u3001\u5408\u6210\u81f4\u6b7b\u7387\u548c\u87a2\u5149\u5831\u544a\u7b49\u65b9\u6cd5\u3002<\/p>\n\n\n\n

\u8fd1\u5e74\u4f86\uff0c\u96a8\u8457\u55ae\u7d30\u80de\u6e2c\u5e8f\u7684\u666e\u53ca\uff0c\u5c07\u5176\u8207\u6df7\u5408\u57fa\u56e0\u5243\u9664\u7be9\u9078\u6cd5\u76f8\u7d50\u5408\uff0c\u5be6\u73fe\u5168\u8f49\u9304\u7d44\u7bc4\u570d\u5167\u7684\u5b9a\u91cf\u5206\u6790\uff0c\u5927\u5927\u63d0\u5347\u7be9\u9078\u7684\u901a\u91cf\uff0c\u4f46\u662f\uff0c\u9019\u4e9b\u65b9\u6cd5\u4ecd\u7136\u4e0d\u80fd\u5c0d\u4e9e\u7d30\u80de\u5c64\u9762\u7684\u8868\u578b\u9032\u884c\u5206\u6790\uff0c\u5982\u5728\u6210\u50cf\u5c64\u9762\u80fd\u5920\u89c0\u5bdf\u5230\u7684\u5206\u8fa8\u7387\u4e0b\u7684\u8868\u578b<\/strong>\u3002\u56e0\u6b64\uff0c\u5c07\u5f59\u7e3d\u7684CRISPR\u57fa\u56e0\u7be9\u9078\u8207\u7d30\u80de\u548c\u4e9e\u7d30\u80de\u6210\u50cf\u8b80\u6578\u76f8\u7d50\u5408\uff0c\u5c0d\u65bc\u6539\u5584\u57fa\u65bc\u5716\u50cf\u7684\u57fa\u56e0\u5243\u9664\u7814\u7a76\u4e2d\u7684\u8868\u578b\u5b9a\u7fa9\u81f3\u95dc\u91cd\u8981\u3002<\/p>\n\n\n\n

2020\u5e745\u670811\u65e5\uff0c\u52a0\u5dde\u5927\u5b78\u8056\u5730\u4e9e\u54e5\u5206\u6821\u7684\u83ef\u88d4\u5b78\u8005Gene W Yeo\u4ee5\u53ca\u5176\u4ed6\u540c\u4e8b\u5728Neture methods\u96dc\u8a8c\u4e0a\u767c\u8868\uff0c\u984c\u76ee\u70baPooled CRISPR screens with imaging on microraft arrays stress granule factors\u7684\u7814\u7a76\u8ad6\u6587\u3002<\/p>\n\n\n\n

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\"\"<\/figure>\n<\/figure>\n\n\n\n
<\/div>\n\n\n\n

\u5728\u9019\u7bc7\u6587\u7ae0\u4e2d\uff0c\u4f5c\u8005\u5c07Pooled CRISPR-Cas9\u7be9\u9078\u8207\u57fa\u56e0\u82af\u7247\u6280\u8853\u4ee5\u53ca\u9ad8\u8996\u93e1\u6210\u50cf\u7d50\u5408\uff0c\u958b\u767c\u65b0\u6280\u8853”CRaft-ID”<\/strong>\u3002<\/p>\n\n\n\n

\u5728\u5927\u91cf\u6210\u50cf\u61c9\u7528\u4e2d\uff0c\u986f\u5fae\u93e1\u5149\u6e90\u9700\u8981\u5177\u6709\u6975\u4f73\u7684\u5149\u6e90\u5f37\u5ea6\u548c\u9577\u6642\u9593\u5de5\u4f5c\u7684\u7a69\u5b9a\u6027<\/strong>\uff0c\u5b8f\u8679CELESTA\u5149\u6e90\u6eff\u8db3\u6240\u6709\u56b4\u82db\u8981\u6c42\u3002CELESTA\u5149\u6e90\u69cb\u5efa\u7684CRaft-ID\u7be9\u9078\u5e73\u53f0\u62d3\u5c55\u4e86CRISPR\u7be9\u9078\u5728\u9ad8\u8996\u93e1\u6210\u50cf\u4e2d\u7684\u61c9\u7528\uff0c\u4f7f\u5f97\u6211\u5011\u80fd\u5920\u5c0d\u61c9\u4e9e\u7d30\u80de\u5c64\u9762\u548c\u7d30\u80de\u578b\u614b\u7684\u8868\u578b\u9032\u884c\u907a\u50b3\u56e0\u5b50\u5206\u6790\uff0c\u800c\u9019\u4e9b\u5728\u4ee5\u524d\u662f\u7121\u6cd5\u5be6\u73fe\u7684\u3002\u7531\u65bc\u8a72\u65b9\u6cd5\u63a1\u7528\u7684\u57fa\u56e0\u82af\u7247\u3001\u901a\u7528CRISPR\u8cc7\u6599\u5eab\u3001\u73fe\u6210\u5171\u805a\u7126\u6210\u50cf\u6280\u8853\u548cPCR\u7684DNA\u6e2c\u5e8f\uff0c\u56e0\u6b64\u5177\u6709\u5ee3\u6cdb\u61c9\u7528\u524d\u666f\u3002<\/p>\n\n\n\n

CELESTA\u5149\u6e90\u4ecb\u7d39<\/h2>\n\n\n\n
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  • CELESTA\u5305\u542b4~7\u53ef\u55ae\u7368\u63a7\u5236\u7684\u56fa\u614b\u6fc0\u5149\u6392\u5217<\/li>
  • \u652f\u6301\u5feb\u901f\u5207\u63db<\/li>
  • \u5149\u5f15\u64ce\u5728\u76f4\u5f911.5mm\u5149\u7e96\u7aef\u51fa\u5149<\/li>
  • \u6bcf\u500b\u6fc0\u5149\u5668\u80fd\u63d0\u4f9b\u7d041W\u7684\u8f38\u51fa\u529f\u7387<\/li>
  • \u63d0\u4f9b4\u62165\u901a\u9053\u9078\u64c7<\/li><\/ul>\n<\/div>\n\n\n\n
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    \"\"<\/figure>\n<\/figure>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
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    Contact Us<\/h2>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
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    \"\"<\/figure>\n<\/figure>","protected":false},"excerpt":{"rendered":"

    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